Single-cell level assay of protein biosynthesis and degradation

By: James V. Kohl | Published on: December 4, 2014

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry

Excerpt: “We use this technique to show modulation of a microRNA critical for T-cell function, miR-155. We adapt this assay for simultaneous detection of mRNA and proteins by ImageStream technology.”
Reported as:

Technology breakthrough reveals cellular transcription process

My comment: Their assay simultaneously detects epigenetically-effected changes in the miRNA/mRNA balance and proteins, which link nutrient-uptake to the physical landscape of DNA in the organized genomes of species from microbes to man.At the single-cell level, they are beginning to assess how RNA-directed DNA methylation and RNA-mediated events link bio-physically constrained thermodynamic cycles of protein biosynthesis and degradation to “…translational and expressional effects in both normal and pathologic conditions.”
They have moved beyond what already is available to link metabolic networks and genetic networks in the context of pharmacogenomics, which enabled serious scientists to distinguish the difference between effects of mutations that perturb protein folding and RNA-mediated amino acid substitutions that stabilize DNA in organized genomes via fixation in the context of pheromone-controlled reproduction.

I had hoped that this breakthrough would link nutrient-dependent RNA-directed DNA methylation from RNA-mediated events and amino acid substitutions to cell type differentiation via epigenetic effects on the microRNA/messenger RNA balance in species from microbes to man. Predictably, that will be the next step forward via use of their technique, which indirectly offers yet another refutation of evolutionary theories about beneficial mutations.
For examples see: Nutrient-dependent/pheromone-controlled adaptive evolution: a model
Excerpt: “..the epigenetic ‘tweaking’ of the immense gene networks that occurs via exposure to nutrient chemicals and pheromones can now be modeled in the context of the microRNA/messenger RNA balance, receptor-mediated intracellular signaling, and the stochastic gene expression required for nutrient-dependent pheromone-controlled adaptive evolution.”
See also:
From Fertilization to Adult Sexual Behavior (1996)
Clinically Actionable Genotypes Among 10,000 Patients With Preemptive Pharmacogenomic Testing (2013)

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