Epigenetically effected energy-dependent fluorescence

By: James V. Kohl | Published on: November 13, 2016

See also: Energy-dependent natural florescence and bioluminescence
Membrane vesicles in sea water: heterogeneous DNA content and implications for viral abundance estimates
Published online November 8, 2016, which was day two of a conference held in an attempt to revise neo-Darwinian pseudoscientific nonsense, and also the second day that this block site was disabled.
Abstract excerpt: “…DNA is packaged heterogeneously within vesicle populations, and it appears that vesicles are likely to be a minor component of SYBR-visible particles in natural sea water compared with viruses. Consistent with this hypothesis, chloroform treatment of coastal and offshore seawater samples reveals that vesicles increase epifluorescence-based particle (viral) counts by less than an order of magnitude and their impact is variable in space and time.
My comment: “…fluorescence-based particle (viral) counts” link the sun’s anti-entropic virucidal energy from Schrodinger’s (1944) claims in “What is life?” to all nutrient energy-dependent biophysically constrained RNA-mediated protein folding chemistry. RNA-mediated amino acid substitutions link supercoiled DNA to protection from virus-driven energy theft. That protection links the nutrient-dependent physiology of reproduction to all healthy longevity and all biodiversity. It links viral latency from all ecological variations to all ecological adaptations. It also links this confusing claim to an attempt to place viral latency back into the context of mutation-driven evolution, a ridiculous theory.
See for example: Virus-mediated archaeal hecatomb in the deep seafloor

We estimated that viral infections were responsible for the abatement of 1.0 to 2.2% day−1 (on average 1.6% day−1) of the bacterial abundance and 2.3 to 4.3% day−1 (on average 3.2% day−1) of the archaeal abundance in deep-sea sediments (Fig. 6).

See: Epigenetics and Genetics of Viral Latency

 “… viral latency is responsible for life-long pathogenesis and mortality risk…”

My comment: That fact clearly links the nutrient energy-dependent pheromone-controlled physiology of reproduction in bacteria from ecological variation to ecological adaptation in all living genera. Viral latency is biophysically constrained in bacteria and eukaryotes at a higher rate than in archaea, which is why the biophysical constraints have been linked from RNA-mediated protein folding chemistry to healthy longevity via protection from virus-driven energy theft.
See also: Quantification of Viral and Prokaryotic Production Rates in Benthic Ecosystems: A Methods Comparison
On November 8, 2016 in the conclusion of Membrane vesicles in sea water: heterogeneous DNA content and implications for viral abundance estimates, the authors asked:

Does vesicle DNA impact estimates of viral concentrations in marine ecosystems?

My comment: The biggest fear shared by any biologically uninformed scientist is the truth that Louis Pasteur expressed when he said that “Chance favors the prepared mind.” That claim can be linked to the raw insight expressed by Lewis Thomas, when he wrote: “I should think we might fairly gauge the future of biological science, centuries ahead by estimating the time it will take to reach a complete comprehensive understanding of odor. It may not seem a profound enough problem to dominate all the life sciences, but it contains, piece by piece, all the mysteries” (p. 732).  — Lewis Thomas as cited in The Scent of Eros: Mysteries of Odor in Human Sexuality.
Earlier today, Peter Berean asked how many science articles I read each week and asked about my access to them. This is one that I requested but did not receive, but I found access to it in my search for more information on fluorescence. My point is, the access does not matter, you must first learn what to look for. That is the sign that you have a “prepared mind.”
See: The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice

To identify new factors required for HR-mediated DSB repair, we screened a short interfering RNA (siRNA) library targeting putative members of the ubiquitin E3 ligase family in U2OS cells that stably coexpress green fluorescent protein (GFP)–Rad52 and red fluorescent protein (RFP)- histone 2B (H2B; Fig. 1a)14.

Excerpt 2 (with my emphasis):

Supplementary Figure 1 The RNF138 amino acid sequence is conserved. (A) Amino acid alignment of RNF138 from different organisms is shown. Alignments was performed using Uniprot online tools. Identical residues are highlighted in black, conserved hydrophobic resides in light grey, other conservative changes are shown in darker grey. Star symbols indicate the conserved residues.

My comment: The conserved amino acid sequence has been linked from energy-dependent cell type differentiation to supercoiled DNA in all living genera via their physiology of reproduction and chromosomal rearrangements have been linked from supercoiled DNA to all energy-dependent biodiversity. That fact was reported in 1973.
For instance:

…the so-called alpha chains of hemoglobin have identical sequences of amino acids in man and the chimpanzee, but they differ in a single amino acid (out of 141) in the gorilla (p. 127).”

See also: The secret to safe DNA repair

When a piece of DNA suffers a break in both of its strands, it is repaired through a process called ‘error-free repair pathway’, which, in essence, allows the broken strand to replicate the missing sequence from an intact strain of DNA.

My comment: When you learn what goes wrong, you must try to find out how to link healthy longevity to what goes right. That is the only way I know to avoid the disastrous results of being taught to believe in ridiculous theories.
The secret to safe DNA repair is nutrient energy-dependent, and the stability of organized genomes is controlled by the physiology of reproduction. That fact has been ignored since we first put it into the context of the molecular epigenetics section of this 1996 Hormones and Behavior review. From Fertilization to Adult Sexual Behavior
We didn’t think there would ever be a need to stress the fact that biophysically constrained RNA-mediated protein folding chemistry is nutrient energy-dependent. Indeed, in 1991, Roger Penrose wrote: “How often do we still hear that quantum effects can have little relevance in the study of biology, or even that we eat food in order to gain energy?”(Roger Penrose 8 August 1991).
Since then, there has never been another model of RNA-mediated cause and effect that linked nutrient-dependent pheromone-controlled cell type differentiation in species from microbes to humans. That suggests what went wrong when this patent application was reported in the context of a “billion dollar baby.” RNA interference is too risky to be used as a therapy in most patients. The side effects could also kill us all, if the virus used adapted to one host and spread to others. That fact explains why this patent application was ignored. Simply put, some researchers claimed that the rewards of the treatment would be worth the risk. For comparison, see this non-invasive treatment for the induction of neural stem cells.
Pheromones and the luteinizing hormone for inducing proliferation of neural stem cells and neurogenesis

The present invention provides a method of increasing neural stem cell numbers or neurogenesis by using a pheromone, a luteinizing hormone (LH) and/or a human chorionic gonadotrophin (hCG). The method can be practiced in vivo to obtain more neural stem cells in situ, which can in turn produce more neurons or glial cells to compensate for lost or dysfunctional neural cells. The method can also be practiced in vitro to produce a large number of neural stem cells in culture. The cultured stem cells can be used, for example, for transplantation treatment of patients or animals suffering from or suspected of having neurodegenerative diseases or conditions.

My comment: The cure for all neurodegenerative diseases has since been linked from virus-driven energy theft in archaea to Zika virus-damaged DNA via the transgenerational epigenetic inheritance that links the virus to craniofacial abnormalities and damage to brain development in infants.
The virus-driven energy theft is tractable across every cell type in every individual of every species via observable changes in fluorescence in bacteria and in dinosaur osteocytes. Peter Berean and Larry Kisner Sr., are among the top contenders for the theft of my model and presentation of it as their own. This post is a preemptive strike against their plagiarism.
See also: Epigenetically effected energy-dependent fluorescence (2) 

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