Your indifference is killing you and others (3)

By: James V. Kohl | Published on: August 15, 2017

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Summary: The speed of light on contact with water links the creation of microRNAs to Optical control of membrane tethering and interorganellar communication at nanoscales MicroRNA flanking sequences are linked to all biodiversity via hydrogen-atom transfer in DNA base pairs in solution. The physiology of reproduction links energy-dependent changes in the microRNA/messenger RNA balance to the fixation of amino acid substitutions in supercoiled DNA. Supercoiled DNA protects all organized genomes from the virus-driven degradation of messenger RNA.
See also: Combating Evolution to Fight Disease
See also: Biblical Genesis
…in the Garden of Eden but instructs them not to eat the fruit of the tree of knowledge of good and evil.

Tree of the knowledge of good and evil

…when God forbids the man to eat from that particular tree, he says that if he does so, he is “doomed to die”.
I am fascinated by the facts that link the Biblical metaphors from eating a virus-infected “fruit” to the death of everyone who will ever live and die. That fact can be placed into this context: Past 5,000 years prolific for changes to human genome.

Of 1.15 million single-nucleotide variants found among more than 15,000 protein-encoding genes, 73% in arose the past 5,000 years, the researchers report. 164,688 of the variants — roughly 14% — were potentially harmful, and of those, 86% arose in the past 5,000 years.

See also: Chromogenic In Situ Hybridization Methods for microRNA Biomarker Monitoring of Drug Safety and Efficacy
Reported by Exiqon as: Robust one-day ISH protocol for disease microRNA biomarker development

ISH of biopsy material has become routine pathology procedure for monitoring gene expression and sample characterization. This could be of microRNAs which prove excellent as biomarkers for disease diagnosis, patient stratification and treatment efficacy.

A fast and robust microRNA ISH protocol well-suited for clinical biomarker studies has just been published in Methods Mol Biol using DIG or FAM double-labeled microRNA-specific LNA™-enhanced detection probes in formalin-fixed, paraffin-embedded (FFPE) tissue sections.

Monitoring gene expression and sample characterization via natrurally occuring microRNA biomarkers is the key to developing biomarkers for use in “…disease diagnosis, patient stratification and treatment efficacy.”
The microRNAs need only be linked from flanking sequences to all biodiversity via hydrogen-atom transfer in DNA base pairs in solution and the physiology of reproduction, which links energy-dependent changes in the microRNA/messenger RNA balance from the fixation of amino acid substitutions in supercoiled DNA to protection from the virus-driven degradation of messenger RNA.
See: Optical control of membrane tethering and interorganellar communication at nanoscales

All the observed interactions seem to require the presence of at least two consecutive positively charged residues (‘KK’ or ‘KR’). Replacement of a single lysine with alanine in such dyad motifs could lead to pronounced reduction in puncta formation; whereas KK-to-AA substitutions often abrogate the PB-lipid/PM associations (Fig. 4 and S4†). Further structural studies on the interaction between PB domains and PIs will likely provide a definitive answer by pinpointing the exact molecular determinants.

The temporal and spatial control that connects subcellular compartments to interorganellar communication, which links energy-dependent changes from angstroms to ecosystems in all living genera is RNA-mediated and biophysically constrained by the pheromone-controlled physiology of reproduction. The temporal and spacial control is linked to our visual perception of mass and energy via the sense of smell in bacteria and RNA-mediated amino acid substitutions in the organized genomes of all living genera.
See also: Mechanism of metabolic puncta formation and its functional significance

Many metabolic enzymes have recently been shown to assemble into multi-component puncta in cells, and this assembly is though to play some role in controlling metabolic flux. For example, substrate channeling within these structures could play an important role in metabolism, enabling intermediates to be directly fed from one enzyme to another without dilution. Therefore the assembly of enzymes into these puncta could be an important mechanism for cells to manipulate their metabolism in response to different signals. However, the mechanism of formation of these puncta is completely unknown.

If the energy-dependent formation of these puncta was not known to be linked from differences in the microRNA/messenger RNA balance to the pheromone-controlled physiology of reproduction in all living genera, I could not have found the obvious link from food energy to all biodiversity in this PubMed search.
puncta formation microRNA

For example, food energy is the obvious link from changes in chirality to autophagy. Autophagy protects all organized genomes from the virus-driven degradation of messenger RNA. Simply put, biophysically contrained energy-dependent autophagy protects all living genera from the mutations that serious scientists have linked to all pathology.
See also:
MicroRNA-141 Targets Sirt1 and Inhibits Autophagy to Reduce HBV Replication.

The HIF-2α-MALAT1-miR-216b axis regulates multi-drug resistance of hepatocellular carcinoma cells via modulating autophagy.

MiR-18a upregulation enhances autophagy in triple negative cancer cells via inhibiting mTOR signaling pathway.

Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway.

MiR-20a and miR-20b negatively regulate autophagy by targeting RB1CC1/FIP200 in breast cancer cells.

A membrane microdomain-associated protein, Arabidopsis Flot1, is involved in a clathrin-independent endocytic pathway and is required for seedling development.

Facilitated ethanol metabolism promotes cardiomyocyte contractile dysfunction through autophagy in murine hearts.

C. elegans Dicer interacts with the P-granule component GLH-1 and both regulate germline RNPs.

For comparison, see: “For the past century some people have regarded natural selection as “that secular God.” Now, that title belongs to CRISPR. “— Jay R. Feierman
Feierman has not learned that natural selection for energy-dependent codon optimality links a light-activated endogenous substrate from the innate immune system in bacteria: CRISPR, to the biophysically constrained physiology of pheromone-controlled reproduction in all living genera. That fact is proof of God’s energy-dependent Creation. The claim that the title of “secular God” belongs to CRISPR is as ridiculous as this claim.
7/25/13
Jay R. Feierman: Variation is not nutrient availability and the something that is doing the selecting is not the individual organism. A feature of an educated person is to realize what they do not know. Sadly, you don’t know that you have an incorrect understanding [of] Darwinian biological evolution.
See also: 7/26/13
Jay R. Feierman: I am absolutely certain that if you showed this statement to any professor of biology or genetics in any accredited university anywhere in the world that 100% of them would say that “Random mutations are the substrate upon which directional natural selection acts” is a correct and true statement.
CRISPR is the only obvious link from energy-dependent changes in chirality to autophagy, which protects all organized genomes from the theft of quantized information that links mutations to all pathology via the degradation of messenger RNA.
See also: Your indifference is killing you and others (4)