Cryo-EM from atoms to ecosystems

(click to enlarge)
Summary: I’m not going to continue repeating myself indefinitely. See: Reduced protein synthesis in schizophrenia patient-derived olfactory cells
The reduced protein synthesis is a clear link from the virus-driven degradation of messenger RNA to all pathology.
October 5, 2017 Lactate Metabolism in Human Lung Tumors
Reported as: Study challenges long-standing concept in cancer metabolism
“We were completely shocked by our findings,” said Dr. Ralph DeBerardinis, Professor at CRI, Director of CRI’s Genetic and Metabolic Disease Program, and Chief of the Division of Pediatric Genetics and Metabolism at UT Southwestern. “The oldest observation in cancer metabolism, the Warburg effect, says that lactate is a waste product of the tumor. This concept has driven the vast amount of research in the field. Our finding is a fundamental change in how we think about tumor metabolism.”
The fact that these so-called experts know so little about metabolism and cancer can be viewed in the context of what is known about all the links from energy-dependent changes in atoms to ecosystems in all living genera.
See: Atomic structure reveals how cells translate environmental signals
The team identified an amino acid sequence in the leaflet that is conserved in parasites, suggesting structural insights that may assist in drug discovery for these devastating conditions.
See, for instance: The Supraspliceosome – A Multi-Task Machine for Regulated Pre-mRNA Processing in the Cell Nucleus
See also: Mitochondrial ribosome assembly in health and disease
There is an obvious need to link the conserved amino acid sequence in parasites to healthy longevity via mitochondrial ribosome assembly and from the virus-driven energy theft of quantized energy as information to all pathology via cryo-EM.See: Inching toward the 3D genome
Re: “…the nucleome structure changes as cells age, differentiate, and divide, and researchers want to understand how and why.”
Cell type differentiation is nutrient-dependent. RNA-directed DNA methylation links RNA-mediated amino acid substitutions to cell type differentiation via protein folding during life history transitions. Amino acid substitutions stabilize protein folding; mutations perturb it, during nutrient-dependent theromodynamic cycles of protein biosynthesis and degradation.
Life is physics and chemistry and communication — https://dx.doi.org/10.1111/nyas.12570
The metabolism of nutrients links metabolic networks to genetic networks via species-specific pheromones that control the physiology of reproduction. Simply put, pheromones link nutrient-dependent life via physics, chemistry, and the conserved molecular mechanisms of communication in species from microbes to man.
Until nutrient-dependent protein folding is linked via the conserved molecular mechanisms of amino acid substitutions and pheromone-controlled DNA stability in organized genomes, which links the epigenetic landscape to the physical landscape of DNA, researchers must take a piece-meal approach to integrating the requirements for life and successful life history transitions — despite the fact that life history transitions have been detailed in the context of the honeybee model organism. See: Honey bees as a model for understanding mechanisms of life history transitions https://www.ncbi.nlm.nih.gov/pubmed/15925525
I’m not going to continue repeating myself, indefinitely.
See: Reduced protein synthesis in schizophrenia patient-derived olfactory cells
The reduced protein synthesis is a clear link from the virus-driven degradation of messenger RNA to all pathology
For comparison see:
Re: Flaming by Glen Sizemore on the evolutionary-psychology group msg 89218
Jay R. Feierman: Being nasty and unkind is not a good strategy in an academic discussion or in human relations in general.”
Glen Sizemore: Yeah…but this isn’t a discussion. It is me ridiculing your hopelessly-moronic views.
Jay R. Feierman [NEW]: The problem is that your unkind and nasty remarks are not just directed at me. When you used to be on the human ethology group before you were removed they were directed at others as well. As moderator of the human-ethology group, I used to not post many of your comments for flaming related to many different people. On the evolutionary-psychology group, where Robert Karl Stonjek appears to have much more tolerance for flaming, your unkind and nasty comments are directed toward many other people, including Robert Karl Stonjek, the moderator. Life is too short to deal with people like you. I prefer to deal with nice people in my life and try to surround my self with nice and kind people. As a result, even though you often bring up interesting issues in your postings related to what I’ve posted, I am no longer going to respond to anything you write.
My response:89219
Please place your comments about me into the context of any “nasty” comment by others.
For example: Jay R. Feierman: Variation is not nutrient availability and the something that is doing the selecting is not the individual organism. A feature of an educated person is to realize what they do not know. Sadly, you don’t know that you have an incorrect understanding [of] Darwinian biological evolution. https://groups.yahoo.com/neo/groups/human-ethology/conversations/topics/48182
My interpretation: You called me a biologically uniformed science idiot.
See also: Jay R. Feierman: I am absolutely certain that if you showed this statement to any professor of biology or genetics in any accredited university anywhere in the world that 100% of them would say that “Random mutations are the substrate upon which directional natural selection acts” is a correct and true statement. https://groups.yahoo.com/neo/groups/human-ethology/conversations/topics/48229
My interpretation: You claimed that every professor of biology or genetics in any accredited university anywhere in the world knew more than me about my established area of expertise: RNA-mediated cell type differentiation.
The final solution???
Excerpt: Emerging evidence suggests that ecological heterogeneity across space can influence the genetic structure of populations, including that of long-distance dispersers such as large carnivores.
My comment: Indeed, the emerging evidence actually refutes all the neo-Darwinian nonsense of population genetics . . . etc., etc.
[MODERATOR NOTE: I’m not going to post more from Kohl until he answers the very direct and simple question posed to him by anon, which is whether he (Kohl) believes that RNA splicing can change DNA.]
10/6/17
Until today, I did not respond to Feierman’s hopelessly-moronic question about my beliefs.
He continued to edit or block any attempts to promote intelligent discussion in the Human Ethology Yahoo Group. And finally, after the 2017 Nobel Prize in Chemistry was awarded to the researchers who developed the cryo-EM tecnology, I can say this:
The fact that alternative splicings of pre-mRNA causes changes in DNA was central to the facts about cell type differentiation we placed into the context of the molecular epigenetics section of this 1996 Hormones and Behavior review: From Fertilization to Adult Sexual Behavior https://www.hawaii.edu/PCSS/biblio/articles/1961to1999/1996-from-fertilization.html
Feierman did not post my reply to the question about alternative splicings of pre-mRNA. Instead, he is continuing to ignore everything known to serious scientists about biologically based cause and effect, or to present it in a series of disparate attempts to obfuscate the facts about energy-dependent top-down causation and the pheromone-controlled physiology of reproduction, which helps to biophysically constrains the transgenerational epigenetic inheritance of all diseases.
Today he posted this link to the Human Ethology Yahoo Group
Smell or vision? The use of different sensory modalities in predator discrimination
The information animals obtain about potential predation risk may differ qualitatively depending on the sensory modality by which a cue is perceived.
Facts about multisensory integration were published as:
Global Sensory Impairment Predicts Morbidity and Mortality in Older U.S. Adults
and Olfaction Warps Visual Time Perception
Feierman also posted a link to: Local mating markets in humans and non-human animals
Biological market theory (BMT), which deals with cooperation among unrelated agents in general, is combined with sexual selection theory (SST), which deals with reproductive cooperation, to focus on an aspect that received little attention in the vast SST literature: adaptations that improve mating success when the market value of an individual varies considerably from one local mating market (LMM) to the next.
This, too: Divergence of developmental trajectories is triggered interactively by early social and ecological experience in a cooperative breeder
We found that individuals did specialize in distinct behavioral competences, which led to either delayed dispersal or early independent breeding. The divergence into different behavioral trajectories became apparent only by manipulating both early social and ecological experiences, highlighting the importance of multivariate influences on the development of social trajectories.
There are many different ways to claim that what organisms eat leads to the production of species-specific behaviors via ecological variation that must be linked to the pheromone-controlled physiology of reproduction. Feierman, and others like him, will continue to cite works that obfuscate the facts about cause and effect that have been known to all serious scientists since the time that McEwen et al., (1964) linked the creation of energy from the creation of ATP to the creation of RNA and all biophysically constrained biodiversity.
See: Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of “oxidative phosphorylation”
The synthesis of RNA in isolated thymus nuclei is ATP dependent.
Feierman, and all other biologically uninformed science idiots are not going to admit that they were wrong about everything they ever claimed.
“A new scientific truth does not triumph by convincing its opponents and making them see the light, but rather because its opponents eventually die, and a new generation grows up that is familiar with it.”
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3 MIN READ
0
The stability of organized genomes (3)
3 MIN READ
0
The stability of organized genomes
5 MIN READ
0
Viruses, amino acids, and somatic cell types (3)
9 MIN READ
0
Viruses, amino acids, and somatic cell types (2)
5 MIN READ
0
RNA-mediated morphological AND behavioral phenotypes
2 MIN READ
0
Becoming biologically informed (3)
3 MIN READ
0
Easy editing: Reinventing our RNA world
3 MIN READ
0
Riding the wrong direction
3 MIN READ
0
RNA-mediated gene duplication, fixation, and ecological adaptation
6 MIN READ
0
MicroRNA controlled growth and brain development
7 MIN READ
0
MicroRNA – controlled ecological adaptations
5 MIN READ
0
“New” epigenetic mechanism for lifelong learning?
3 MIN READ
0
microRNAs, glycosylation, and genomes
4 MIN READ
0
Unknown mechanisms and conclusions
2 MIN READ
0
Alternative splicings: epigenetics meets pharmacogenomics
4 MIN READ
0
Epigenetic regulation of aging by glycine and GnRH
5 MIN READ
0
Pattern recognition: biogeochemical structure and function
5 MIN READ
0
Viruses and the human-like microbiome
3 MIN READ
0
Informing the biologically uninformed
< 1 MIN READ
0
I forgot. How do mutations cause evolution?
4 MIN READ
0
Missing a fact: microRNAs are genomic biomarkers
4 MIN READ
1
Ignoring systems complexity (it’s too complicated)
3 MIN READ
0
Computing via phosphorylation and fixation
3 MIN READ
0
A special issue on nutritional epigenetics
5 MIN READ
0
Epigenetics: microRNAs effect an integrative pathway
4 MIN READ
0
MicroRNAs and the exposome
5 MIN READ
0
Linking the origin of birds to dinosaurs
< 1 MIN READ
0
RNA-mediated silencing of a chromosome
3 MIN READ
0
RNA-mediated cell types and precision medicine
5 MIN READ
0
Amino acid-dependent cell type differentiation
8 MIN READ
0
Mutisensory integration: watching the paradigm shift
12 MIN READ
0
Viruses and ecologically adapted animals
2 MIN READ
0
Questions about life’s diversity
21 MIN READ
0
Two types of microRNA are not double agents
2 MIN READ
0
Epigenetics Skeptism
5 MIN READ
0
What about birds?
5 MIN READ
0
Rejecting what is known about viral microRNAs and nutrient-dependent microRNAs
2 MIN READ
0
An epigenetic trap (the prequel)
5 MIN READ
0
Nutritional epigenetics, exercise, and immune system integrity
4 MIN READ
0
Are viruses microRNAs? (2)
5 MIN READ
0
Are viruses microRNAs?
3 MIN READ
0
Sneaking up from behind
4 MIN READ
0
From Hydra to humans vs a Lakatosian research program
4 MIN READ
0
The future of physics predicts no future for evolutionary theory
3 MIN READ
0
Dual genomes: exposing the evolution industry
5 MIN READ
0
Epigenetic pharmacology and RNA-mediated transciptional landscapes
3 MIN READ
0
More than a bag of chemicals?
2 MIN READ
0
Intelligent viruses and cancers?
10 MIN READ
0
RNA eclipses the importance of DNA to cell type differentiation
4 MIN READ
0
Pharmacogenomics
3 MIN READ
0
In theory, or supported by experimental evidence?
5 MIN READ
0
It’s cell type differention, not cell fate determination (2)
3 MIN READ
0
Are evolutionary theorists 'nob ends'?
5 MIN READ
2
No excuses: Creation and the meaning of organismal complexity
7 MIN READ
0
No understanding of biodiversity
2 MIN READ
0
Behavioral ecology: please continue to believe in our fantasies
3 MIN READ
0
Color vision refutes the evolutionary dogma of gene duplication
4 MIN READ
0
Genomic surveillance ends our world of RNA-mediated ecological adaptations
2 MIN READ
0
RNA-mediated cell type differentiation and behavior
3 MIN READ
0
Physics denied; pseudoscientific nonsense accepted
2 MIN READ
0
Stop evolutionary theorists. Kill cancers
3 MIN READ
0
RNA-directed DNA methylation and RNA-mediated events
5 MIN READ
1
Miracles are not miracles to evolutionary theorists
2 MIN READ
0
Seemingly futile cycles are not thermodynamically futile
3 MIN READ
0
“Kardashians” in science
< 1 MIN READ
0
Can epigenetic inheritance occur without concurrent changes in morphology AND behavior?
3 MIN READ
0
Watching and waiting for more retractions
2 MIN READ
0
RNA-mediated events found everywhere
2 MIN READ
0
Genotype, observed phenotype, and distinctly disordered behaviors
3 MIN READ
0
RNA-mediated ecological adaptation is not evolution
2 MIN READ
0
Exploding genomes and chromosomal rearrangements via RNA-mediated events
2 MIN READ
0
Behavior (2): All responses are RNA-mediated not genetically-determined
4 MIN READ
0
Nutrient-dependent erythropoiesis and anemia
6 MIN READ
0
Evolutionary heritage or ecological adaptation? Racism versus reality
4 MIN READ
0
A relatively young branch of science called epigenetics
4 MIN READ
0
Comparing divergent model organisms
2 MIN READ
1
Order and disorder: Ecological adaptations not mutations
6 MIN READ
0
microRNAs and species relationships
3 MIN READ
0
Nutrition, pheromones and cancer (2)
2 MIN READ
0
Not focused and self-aggrandizing
2 MIN READ
0
Cell-type differentiation
2 MIN READ
0
Another powerful refutation of mutation-initiated natural selection
4 MIN READ
0
96 fixed amino acid substitutions, not 96 genes
6 MIN READ
0
Targeting a cancer gene
2 MIN READ
0
Alternative splicings (very technical)
2 MIN READ
0
Social experiences epigenetically effect gene networks
2 MIN READ
0
Pheromone-controlled thermodynamics and cancer
2 MIN READ
0
Evolution: innovations may have non-adaptive origins (sans mutations)
2 MIN READ
0
Pheromones and cancer
4 MIN READ
0