Light-activated PTEN-dependent viral latency (1)
(click to enlarge)
See: Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of “oxidative phosphorylation” (1964)
The synthesis of RNA in isolated thymus nuclei is ATP dependent.
The energy required for biophysically constrained oxidative phosphorylation, viral latency, and cell type differentiation comes from food.PTEN in Chromatin Remodeling 9/30/19 by Yang, J. and Yin, Y.
…we summarize the current understanding of roles of PTEN in controlling chromatin dynamics and global gene expression…
Y. Yin & J. Yang (2019) linked A Quick HYL1-Dependent Reactivation of MicroRNA Production Is Required for a Proper Developmental Response after Extended Periods of Light Deprivation from the light-activated Creation of microRNAs in plants to the ATP-dependent creation of RNA to Yin Yang I as an Epimodulator of miRNAs in the Metastatic Cascade, which links microRNA biogenesis to biophysically constrained viral latency and the genesis of all biodiversity via the physiology of reproduction in all living genera.Spindle-shaped viruses infect marine ammonia-oxidizing thaumarchaea 7/18/19, because:
…viral predation has a profound impact on thaumarchaeal functioning and mortality, thereby regulating global biogeochemical cycles.
More than 55 years after McEwen et al, established the facts about the ATP-dependent creation of RNA, it’s time to move forward. In 2014, I tried, but my invited review of nutritional epigenetics (food energy-dependent microRNA-mediated biophysically constrained viral latency) was returned without review.Nutrient-dependent pheromone-controlled ecological adaptations: from atoms to ecosystems 4/11/14 (preprint)PTENα, a PTEN isoform translated through alternative initiation, regulates mitochondrial function and energy metabolism 4/24/14
Our studies demonstrate the importance of eIF2A-mediated alternative translation for generation of protein diversity in eukaryotic systems and provide insights into the mechanism by which the PTEN family is involved in multiple cellular processes.
The claim that a PTEN isoform regulates energy-dependent mitochondrial function and energy metabolism eliminates the fact that the light-activated assembly of the microRNA-RNA-peptide nanocomplex must be linked from the ATP-dependent creation of RNA to the physiology of reproduction; to biophysically constrained viral latency; and to healthy longevity across all kingdoms. Nutrient-dependent Pheromone-Controlled Ecological Adaptations: From Angstroms to Ecosystems 4/18/18PTENα regulates mitophagy and maintains mitochondrial quality control emphasis )
Damaged mitochondria produce excessive reactive oxygen species, causing DNA damage, lipid and protein oxidation, and ultimately cell death. A series of well-coordinated quality control processes have thus evolved to maintain normal mitochondrial activity.
For comparison, all serious scientists know that the Creation of energy as information regulates mitophagy and the Creation of sunlight is required for mitochondrial quality control via microRNA-mediated autophagy. Why hasn’t that fact always been perfectly clear?Darwin’s Radio , ended with publication of The Complex Landscape of PTEN mRNA Regulation 12/23/19 by Sellars, E.Gabra, M.Salmena, L. (with my emphasis )
Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a key tumor suppressor in the development and progression of different tumor types. Emerging data indicate that small reductions in PTEN protein levels can promote cancer. PTEN protein levels are tightly controlled by a plethora of mechanisms beginning with epigenetic and transcriptional regulation and ending with control of protein synthesis and stability. PTEN messenger RNA (mRNA) is also subject to exquisite regulation by microRNAs , coding and long noncoding RNAs, and RNA-binding proteins. Additionally, PTEN mRNA is markedly influenced by alternative splicing and variable polyadenylation. Herein we provide a synoptic description of the current understanding of the complex regulatory landscape of PTEN mRNA regulation including several specific processes that modulate its stability and expression, in the context of PTEN loss-associated cancers.
The US patent for the energy-dependent microRNA-mediated effective treatment and/or prevention of all cancers was issued on 12/25/18.Methods and compositions for identifying a peptide having an intermolecular interaction with a target of interest
A system of a recombinant bacteriophage library and a target of interest complex, wherein the recombinant bacteriophage peptide library includes a plurality of peptides expressed on the surface of recombinant bacteriophages wherein each recombinant bacteriophage includes (a) a pill protein; wherein each pill protein includes (b) a peptide or polypeptide involved in an intermolecular interaction, which differs by at least one amino acid from other peptides or polypeptides in the library; and (c) a modified protease cleavage site proximal to the peptide, wherein the modified protease cleavage site is the same in each bacteriophage, the modified cleavage site having a reduced binding affinity to a protease, as compared to a non-modified cleavage site, and wherein the target of interest complex includes a protease, a flexible linker attached to the protease, and a target of interest attached to the flexible linker, wherein the target of interest participates in an intermolecular interaction.
See also: Proof of concept
After you realize how important the energy-dependent Creation of PTEN is to biophysically constrained viral latency, see Feynman’s comments on where the energy comes from:VIDEO
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Kohl and Francoeur at 25 (8)
2
0
Light and life-sucking black holes (2)
2
0
The tipping point (revisited): 85,000 publications
4
0
Kohl and Francoeur at 25 (3)
3
0
Biophysical constraints on RNA synthesis (1)
9
0
Preprints obfuscate facts (2)
4
0
RNA processing and disease (3)
3
0
Ecological adaptation vs neo-Darwinian nonsense (1)
2
0
Blood music orchestrates human life (3)
3
0
Do not call me Ishmael (1)
3
0
Phytoremediation, Microbiome and CRISPR (4)
4
0
Phytoremediation, Microbiome and CRISPR (3)
5
0
Autophagy automagically prevents cancer (2)
2
0
Phytoremediation, Microbiome and CRISPR (1)
3
0
Light-activated constrained biodiversity (2)
4
0
Light-activated carbon fixation (2)
3
0
The tipping point (revisited): 82,000 publications (5)
3
0
The tipping point (revisited): 82,000 publications (1)
3
0
Viral latency vs deadly paleoanthropology (1)
4
0
Light and life at base pair resolution (10)
4
0
Plant microRNAs regulate across-kingdom gene expression (1)
2
0
Code Biology vs Predatory Publishing (2)
2
0
Two photon-linked biodiversity (4)
2
0
From microRNA.pro to quantumsouls.pro (1)
4
0
Light and life at base pair resolution (4)
4
0
Hide and seek with science facts (8)
2
0
Hide and seek with science facts (7)
3
0
Hide and seek with science facts (1)
4
0
The perfect symmetry of nature
2
0
Bottom-up biology
3
0
Happy Veteran’s Day 2018 (2)
4
0
SfN’s 2018 trap set for Democrats
2
0
Evolved suffering and death caused by statistical significance
< 1
0
War Games: False Flag Terrorism (4)
3
0
Creating an enzyme that kills theories (1)
< 1
0
Darwin’s Children (2003) and Denton’s Children of the Light (1)
2
0
Biologos bastardized it all, again
3
0
Are Kavanaugh’s accusers terrorists?
4
0
Diagnosis: 120 years of human idiocy
< 1
0
microRNA targeting efficacy refutes ridiculous theories
3
0
Information Wars started by medical practitioners (1)
< 1
0
Natural models inspire future therapeutics (2)
2
0
Quantum initiation of cold chemistry vs Hypeology (2)
3
0
Predicting naturally occurring viral latency
3
0
The concept of a species (3)
4
0
The tipping point (revisited): 74,000 publications
4
0
Laws of Biology vs no Laws of Physics (2)
3
0
MicroRNA-mediated alternative splicing (revisited)
4
0
Animal welfare vs human ethology
4
0
Biologically uninformed science idiot: Self-defense (1)
3
0
The eternal significance of microRNAs and the Vietnam Memorial (2)
4
0
The eternal significance of microRNAs and the Vietnam Memorial (1)
3
0
From quantum physics to quantum souls (2)
2
0
Epigenetic inheritance of spatiotemporal regulation (5)
8
0
The eternal significance of microRNAs (10)
5
0
The eternal significance of microRNAs (7)
3
0
A single base change refutes theistic evolution (2)
2
0
A single base change refutes theistic evolution
5
0
EDAR V370A and sympatric speciation
2
0
Virus-driven cystinosis
2
0
Environmental selection is natural selection (4)
2
0
Environmental selection is natural selection
3
0
Abiogenesis vs microRNA biogenesis (2)
7
0
2018 March for Science vs microRNAs (2)
4
0
2018 March for Science vs microRNAs
7
0
The eternal significance of microRNAs (1)
< 1
0
Sympatric Speciation vs pseudosceintific nonsense (4)
2
0
Complexity: Routes and Patterns (4)
< 1
0
NSF’s parrot and other parrots
2
0
Let there be anti-entropic virucidal light
3
0
RNA-mediated nutritional psychiatry (2)
2
0
RNA-mediated nutritional psychiatry
4
0
Polymaths and paradigm shifts: From Asimov to Bear (5)
5
0
Polymaths and paradigm shifts: from Asimov to Bear (1)
5
0
How to create biologically uninformed theorists
7
0
Carl Zimmer refutes theistic evolution (2)
2
0
Ecological adaptation: A new definition of heredity (3)
4
0
Creating biophysically constrained viral latency (2)
2
0
Light-activated carbon fixation did not evolve (4)
7
0
The tipping point (revisited): 70,000 publications
4
0
Diet-driven RNA interference and mental health (4)
8
0
Cryo-EM: Linking spatial and conformational constraints
9
0
A reversible TCA cycle in a thermophile (3)
4
0
A mental health problem at the highest level (3)
7
0
Diet-driven RNA interference and mental health (3)
4
0
Anti-entropic sunlight: Schrödinger’s Creationist Secret? (4)
8
0
Anti-entropic sunlight: Schrödinger’s Creationist Secret? (3)
6
1
Anti-entropic sunlight: Schrödinger’s Creationist Secret? (2)
4
0
The MicroRNAome Strikes Back: A Sokalian hoax (9)
4
0
BiondVax Universal Flu Vaccine Patent
4
0
The MicroRNAome Strikes Back: A Sokalian hoax (5)
6
0
The MicroRNAome Strikes Back: A Sokalian hoax (4)
5
0
The MicroRNAome Strikes Back: A Sokalian hoax (3)
4
0
The MicroRNAome Strikes Back: A Sokalian hoax (2)
6
0
A mental health problem at the highest level (2)
3
0
Diet-driven RNA interference and cancer prevention (3)
6
0
Enzyme-constrained interethnic diversity (8)
< 1
0
Enzyme-constrained interethnic diversity (7)
6
0
Interethnic genetic similarities and differences (2)
4
0
Enzyme-constrained interethnic biodiversity (1)
5
0
The “walking fish” walks straight from quantum physics to quantum souls (4)
11
0
The “walking fish” walks straight from quantum physics to quantum souls (2)
3
0
A Mathematical Model Links Quantum Physics to Quantum Souls (1)
6
0
From base editing to RNA editing (7)
2
0
From base editing to RNA editing (6)
8
0
Darwinian adaptation: a clear evolutionary trail?
4
0
Agilent technology and energy-dependent autophagy
8
0
Narcissistic egomaniacal and deadly denial of autophagy
6
0
The overwhelming ignorance of sex researchers (2)
4
0
Energy-dependent base editing and correction of mutations (2)
9
0
Trashing the 2nd Law
7
0
The overwhelming ignorance of sex researchers
5
0
Two retractions of human idiocy
2
0
Reporting new scientific truths is not allowed in the USA
3
0
MicroRNAs and the Cassandra syndrome (revisited)
4
0
A null mutation and healthy longevity?
4
0
Light-activated error free DNA repair
5
0
Achromobacter and killer theories
2
0
Eutrophication and phosphorylated biodiversity
6
0
A mental health problem at the highest level
2
0
The next reformation: 500 years too late? (1)
< 1
0
The next reformation: 500 years too late? (2)
2
0
From base editing to RNA editing (2)
7
0
Cryo-EM: More than a suggestion
< 1
0
Exome Sequencing Impact in Routine Care
4
0
Losers: rediscovering an epigenetic key
2
0
Virulence: Out of stock
2
0
Energy-dependent structure and function: Until death (5)
4
0
Energy-dependent structure and function: Until death (3)
3
0
Energy-dependent structure and function: Until death (2)
5
0
Energy-dependent structure and function: Until death (1)
4
0
Predicting who wins the 2017 Nobel Prizes (7)
4
0
Entertaining yourself to death
6
0
Predicting who wins the 2017 Nobel Prizes (6)
5
0
Predicting who wins the 2017 Nobel Prizes (2)
4
0
Predicting who wins the 2017 Nobel Prizes
4
0
Life and death via chemical effects on photosynthesis
8
0
Dispelling the ignorance of theorists
4
0
Sci-Hub vs limits on the dissemination of information
6
0
Evolutionary theories of epigenetic drift
2
0
Host-derived creation of all pathology (2 of 2)
9
0
God vs host-derived creation of virus-driven pathology
6
0
Your indifference is killing you and others (4)
4
0
Robert Sapolsky’s legacy of atheistic pseudoscientific nonsense
4
0
Can protein folding chemistry be understood by theorists?
5
0
Pattern recognition vs evolutionary processes (revisited)
7
0
Energy-dependent physical and biophysical constraints (10)
8
0
God’s shrinking role in salvation (3)
9
0
God's shrinking role in salvation (3)
9
0
God’s shrinking role in salvation (2)
5
0
MicroRNAs light up life
7
0
From E. coli to monkeys and mankind: Theories vs models (5)
5
0
From E. coli to monkeys and mankind: Theories vs models (2)
6
0
Food energy-dependent epigenetic adaptation (2)
< 1
0
Energy-dependent microRNA biogenesis
4
0
The Virus-Driven Suicide March for Science
5
0
Viruses in pathogenic variants disrupt alternative splicings (2)
3
0
Respiration-dependent endogenous RNA interference
4
0
Epigenetically-effected nucleosome repositioning sheds Dobzhansky’s light on evolution
3
0
Functionally interdependent editing and methylation
4
0
Cytosis: Biology Content
7
0
Food for thought: Penrose v Ellis (3)
4
0
Magnussen’s “Faith fools” keep the faith
3
0
The essence of precision medicine
4
0
Allen Institute confirms food energy is information
6
0
RNA-mediated adult learning, memory, and neurogenesis
3
0
Theistic evolutionists fight back and lose
4
0
Bill Gates refutes theistic evolution (sequel)
8
0
Bill Gates refutes theistic evolution
4
0
Francis S. Collins refutes theistic evolution
6
0
Energy is information. Objections over ruled. You’re fired!
7
0
Happy Darwin Day (2017)
4
0
George Church refutes theistic evolution
5
0
Mitochondria do not adapt
2
0
Critical values expose virus-driven energy theft (2)
4
0
Critical values expose virus-driven energy theft
6
0
Energy-dependent hydrogen bonds in supercoiled DNA
7
0
Energy-dependent chirality (2)
3
0
Energy-dependent chirality
6
0
Vietnam Veterans and others with glioblastoma
3
0
Energy-dependent sensory maps (1996-2016)
5
0
Re-inventing mutation-driven evolution (2)
4
0
De novo gene creation: Ignoring the experimental evidence
7
0
Energy-dependent coulombic, autophagic, polycombic healthy longevity
4
0
Energy-dependent purifying selection / autophagy (3)
2
0
Energy-dependent purifying selection / autophagy (5)
2
0
Energy-dependent purifying selection / autophagy
2
0
Epigenetically effected energy-dependent fluorescence
5
0
Energy-dependent polycombic ecological adaptation
21
0
Did evolution autophosphorylate your kinases? (3)
2
1
The natural success of RNAi and failed treatment
7
0
Nutrient-dependent pheromone-controlled autophagy (2)
6
0
Nutrient-dependent pheromone-controlled autophagy
3
0
Life is energy-dependent task management
3
0
Chromatin: The structure of DNA
6
0
Hydrogen-atom energy in DNA base pairs
6
0
Light energy-induced base pair changes (2)
4
0
Light energy-induced base pair changes (1)
6
0
How did the innate immune system evolve?
4
0
The Aquatic Ape: New evidence?
4
0
Protecting 4 billion people from virus-driven entropy
< 1
0
Lawless evolution: No physics
4
0
Antithetical conclusions (7)
3
0
Non-random pheromone-controlled cell type differentiation
3
0
RNAi: From magic bullet to billion dollar baby
4
0
GC-rich neo-Darwinian train wreck
5
0
From angstroms to ecosystems
7
0
Food supplement or licensed immunostimulant?
6
0
Biophotonics, glycobiology, quantized biodiversity (2)
19
0
The end of neo-Darwinism
5
0
Biophotonics, glycobiology, quantized biodiversity
4
0
Did “Nature” kill Steve Jobs? (3)
10
0
Did “Nature” kill Steve Jobs? (2)
6
0
Did “Nature” kill Steve Jobs?
3
0
Placing boundaries where none exist
< 1
0
Innate immunity and multiplexing
7
0
RNA methylation (2)
7
0
Masters of deception about nature (2)
7
0
Cracking the Olfactory Code?
5
0
Energy-dependent RNA methylation (9)
2
0
Energy-dependent RNA methylation (7)
7
0
Energy-dependent RNA methylation (6)
9
0
Energy-dependent RNA methylation (5)
3
0
Virus-driven energy theft: definition?
5
0
From angstroms to ecosystems and entropy
2
0
Nutrient-dependent RNA-mediated cause and effect
3
0
Cancer: Evolution 2.0’s Blind Spot
13
0
MicroRNA-mediated RNA epigenetics
2
0
RNA-mediated theory killers (4)
9
0
An epigenetic trap (the prequel)
5
0
Are viruses microRNAs?
3
0
An epigenetic trap (the sequel)
10
0
We need pattern recognition, not a prologue
6
0
Genotype, observed phenotype, and distinctly disordered behaviors
3
0
Jay R. Feierman
3
0
Human Pheromones and Consilience
2
0
(click to enlarge)