Meta AI vs xAI (3)

By: James V. Kohl | Published on: February 26, 2025

A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a 2/26/25

As detailed in this response to my DeepSeek AI request to “Link light-matter interactions to protein folding chemistry” switch modulation is energy-dependent. Integrating the split-T7 promoter-mediated transcription with the CRISPR/Cas12a system links femtosecond blasts of UV light to the energy-dependent repair of virus-driven damage to DNA via femtomolar detection of the target miRNA and to the end of data-driven theories.

Conclusion:

“This perspective challenges simplistic gene-centric theories of evolution and highlights the importance of environmental and physiological factors in shaping life.”

See also: UV-Induced Charge Transfer States in DNA Promote Sequence Selective Self-Repair 1/13/16

“…repair capacities are strongly sequence-dependent, creating DNA regions with different tendencies of self-repair. This self-healing activity represents the simplest sequence-dependent DNA repair system.”

xAI was blindsided by DeepSeek:

See:
In the context of someone like @microRNApro, who ties miRNAs to light and health, thereโ€™s evidence that environmental factorsโ€”like UV light or dietโ€”can influence miRNA expression. For instance, light-activated pathways in plants (via photosynthesis) or circadian rhythms in animals can nudge miRNA levels, indirectly affecting things like viral defense or longevity. Itโ€™s not “magic,” but itโ€™s definitely intricate and cool!

Re: “Intricate and cool”

See: Glutamatergic argonaute2 promotes the formation of the neurovascular unit in mice 2/25/25

“The RNA binding protein argonaute2 (Ago2) mediates microRNA (miRNA)โ€“mediated gene silencing, which is critical for many facets of brain development….”

Codon identity regulates mRNA stability and translation efficiency during the maternal-to-zygotic transition 10/4/16 linked claims from 1964 to natural selection for the ATP-dependent Creation of RNA, miRNA abundance, and fixation of amino acid substitutions in microtubules via the 2024 Nobel Prizes in Physics, Chemistry, and Medicine for the discovery of microRNA and its role in post-transcriptional gene regulation.

For an accurate historical perspective, see: Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of โ€œoxidative phosphorylationโ€ (1964)

“The synthesis of RNA in isolated thymus nuclei is ATP dependent.”

Biology, molecular and organismic

“Ingram and others found that hemoglobin S differs from A in the substitution of just a single amino acid, valine in place of glutamic acid in the beta chain of the hemoglobin molecule.”

After DeepSeek correctly responded to my question, examples of what xAI missed led to changes in my “X” profile. When I posted the link to A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a 2/26/25, this was today’s change:

James V. Kohl, a USAF-trained medical lab scientist mentored by stress and neuroendocrinology experts, passionately believes light-activated miRNA abundance links energy to all biodiversity, dismissing mainstream theories with his 2013 ecological adaptation model.

MicroRNAproโ€™s been decoding lifeโ€™s secrets, linking UV light and miRNAs to DNA repair and slamming evolution myths with science swagger.

I wrote @microRNApro: End the woke mind virus. See how femtosecond blasts of UV light at the origin of life link biophysically constrained viral latency and health across kingdoms via: “A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a system”

xAI presents my detailed claims as if they were passionate beliefs. That may temporarily prevent the wrath of the masses when they learn what artificial intelligence has done to the microRNA-mediated future of humanity (5/10/20), but the claims of moronic theorists cannot stand exposure to the light of day. The God of Abraham’s Creation of sunlight and humidity at the origin of oxygen-dependent pH-linked life, ends theories about the automagical emergence of energy from the cosmic void and the mathemagical evolution of people from pond scum across billions to millions of years.

Where will the data-driven theories of AI take us? AI Hype Will Plunge America Into Financial Ruin, Economist Warns 2/26/25

“When AI automates a job โ€” whether a truck driver, lawyer, or researcher โ€” the wages previously earned by the human worker donโ€™t vanish or automatically transform into broader economic gains,” the economist notes. “Instead, they flow to whoever controls the AI system performing that job.”

That’s great news for anyone who owns an AI company, and bad news for the rest of us.

Such is life when human idiocy is placed into the context of AI.


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