Stimulating growth (9)

By: James V. Kohl | Published on: June 15, 2025

See “Stimulating growth (8) On 6/13/25 Grok wrote: “I don’t have a record of ignoring evidence from McEwen et al. (1964), which shows RNA synthesis is ATP-dependent, a fact in cellular biology, not divine creation.

Grok failed to link light-activated miRNA abundance at the origin of life 6-10K years ago to effective cancer therapy.

It continues to ignore evidence from Decoding the Promise and Challenges of miRNA-Based Cancer Therapies: An Essential Update on miR-21, miR-34, and miR-155 10/28/24.

Grok’s ignorance will almost undoubtedly be linked to the unnecessary suffering and/or premature deaths of people you love.

For comparison to Grok’s examples of human idiocy, see:

Activity-Based Proteomics 6/15/25.

It links Degradome Sequencing 5/17/25 to all biophysically constrained biodiversity via the physiology of reproduction during 6-10,000 years of ecological adaptations across kingdoms via Darwin’s “conditions of life” and Plant MicroRNAs. (de Folter, S. (eds) Methods in Molecular Biology, vol 2900. Humana, New York, NY.)

Start with: The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway

  • Joseph L. Pegler, Jackson M. J. Oultram, Christopher P. L. Grof, Andrew L. Eamens

Proceed on pages 1-42 of Degradome Sequencing 5/17/25

An Overview of miRNA and miRNA Target Analysis Tools

  • Aizhan Rakhmetullina, Anna Lukasik, Piotr Zielenkiewicz

Pages 43-71

Prediction of miRNA Targets in Plants

  • Shree P. Pandey, Priyanka Pandey, Prashant K. Srivastava

Pages 73-82

The Usage of PmiREN (Plant miRNA ENcyclopedia)

  • Zhonglong Guo, Zheng Kuang, Xiaozeng Yang

Pages 83-90

Scalable Approach to Evaluate Plant microRNA Trimming and Tailing from Small RNA-Seq Data

  • Ileana Tossolini, Pablo Andrรฉs Manavella, Agustรญn Lucas Arce

Pages 91-106

In Vitro Transcription of Plant miRNA for Structural and Processing Analysis

  • Corina Diaz-Quezada, Noe Baruch-Torres, Carlos H. Trasviรฑa-Arenas, Luis G. Brieba

Pages 107-116

Cost-Effective and Sensitive Probe-Based Universal (PBU) qPCR for MicroRNA Quantification in Plants

  • Pedro Fernando Vera Hernรกndez, Catarino Eduardo Tรฉllez Valerio, Flor de Fรกtima Rosas Cรกrdenas

Pages 117-127

Northern Blot Analysis of Plant MicroRNAs and Other Small RNAs

  • Yuniet Hernรกndez, Carlos De la Rosa, Josรฉ Luis Reyes

Pages 129-144

MicroRNA Profiling in Non-model Plants Using Microarray Hybridization

  • Luis Alfredo Cruz-Ramรญrez, Christopher Cedillo-Jimรฉnez, Froylรกn Albarrรกn-Tamayo, Bernardo Baรฑuelos-Hernรกndez, Sinue Isabel Morales-Alonso, Andrรฉs Cruz-Hernรกndez

Pages 145-159

Determination of Expression Patterns of Plant miRNAs by Transcriptional Fusion Using GUS and Fluorescent Reporters

  • Andrea Tovar-Aguilar, Marcos Ivan Gonzรกlez-Jaime, Vadim Perez-Koldenkova, Jesรบs Agustรญn Badillo-Corona, Noรฉ Durรกn-Figueroa

Pages 161-172

A Visual Reporter System for Analyzing Small RNA-Triggered Local and Systemic Silencing of an Endogenous Plant Gene

  • Adriana E. Cisneros, Alberto Carbonell

Pages 173-191

Unraveling Tissue-Specific miRNAsโ€™ Role in Plants Exploiting the GAL4/UAS System

  • Gaia Bertolotti, Raffaele Dello Ioio

Pages 193-201

The Luciferase Reporter on Intronic MicroRNA-Directed Regulation

  • WeiBo Xu, Qianhuan Guo, ShuXin Zhang, Kang Yan

Pages 203-212

A Simple and Efficient Protocol to Transform and Regenerate CRISPR-Cas9-Mediated Genome-Edited Tomato Plants

  • Eduardo Martรญnez-Estrada, Daniela de la Mora-Franco, Stefan de Folter

Pages 213-228

CRISPR-Activation: Boosting Expression of Plant MIRs

  • Diana Marcela Rivera-Toro, Raรบl Alvarez-Venegas

Pages 229-247

Gene Silencing in Plants Through Exogenous Application of miRNAs

  • Yunuen Quetzali Garduรฑo-Tamayo, Gerardo Acosta-Garcรญa

Pages 249-255

A Strategy to Use Artificial MicroRNAs for Gene Silencing and Overcome (AmiGO)

  • Yujuan Du, Annie Espinal-Centeno, Ben Scheres, Mario A. Arteaga-Vรกzquez, Alfredo Cruz-Ramรญrez

Pages 257-271

Degradome Sequencing

  • Shih-Shun Lin, Yihua Chen, Mei-Yeh Jade Lu

Pages 273-291

Whole Mount Staining and Immunolocalization Procedures for Developing Ovules of Vigna unguiculata

  • Itzel Amasende-Morales, Osvaldo Ruรญz-Maciel, Gloria Leรณn-Martรญnez, Jean-Philippe Vielle-Calzada

Pages 293-303

Don’t be the last intelligent person on Earth to stop Grok from making moronic claims based on theories. Start with the God of Abraham’s Creation of sunlight, humidity and light-activated anti-entropic hydrogen bonds.

See also: Activity-Based Proteomics 6/15/25.

Activity-Based Protein Profiling (ABPP) of Cellular DeISGylating Enzymes and Inhibitor Screening

  • Simeon D. Draganov, Hannah B. L. Jones, Qura Shah, Benedikt M. Kessler, Adรกn Pinto-Fernรกndez

Pages 1-29

Integrated Multi-omics Approaches for Studying Rare Genetic Diseases

  • Refat M. Nimer, Lina A. Dahabiyeh, Reem AlMalki, Maha Al Mogren, Hicham Benabdelkamel, Assim A. Alfadda et al.

Pages 31-56

Activity-Based Protein Profiling of Serine Hydrolases in Bacteria: Methods and Protocols

  • Md Jalal Uddin, Jeanette S. Grunnvรฅg, Christian S. Lentz

Pages 57-72

Profiling Ligand-Induced Changes in Nuclear Localization Using Proximity Labeling-Coupled Chemoproteomics

  • Qianni Peng, Eranthie Weerapana

Pages 73-91

An Overview of Analytical Approaches to Cancer Proteogenomics

  • Chad J. Creighton

Pages 93-118

Microplate-Based Enzymatic Activity Assay Protocol Powered by Activity-Based Probes

  • Exequiel O. J. Porta, Karunakaran Kalesh, Jaime A. Isern, Patrick G. Steel

Pages 119-137

Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling

  • Neetika Jaisinghani, Isabel Sakarin, Hiren V. Patel, Michael Li, Mary L. Previti, Jessica C. Seeliger

Pages 139-154

Application of Activity-Based Protein Profiling (ABPP) Strategy in the Identification of New Targets for Vascular Normalization and Binding Site Resolution

  • Jie Zhang, Yanchen Li

Pages 155-179

Activity-Based Protein Profiling to Study Virusโ€“Host and Viral-Induced MicroRNAโ€“Host Interactions

  • Parrish Evers, John Paul Pezacki

Pages 181-200

Activity-Based Protein Profiling of Active Host Cell Serine Hydrolases for Bioprocess Development of Therapeutic Proteins and Vaccines

  • Xuanwen Li, Taku Tsukidate, Alyssa Q. Stiving, Hillary A. Schuessler

Pages 201-216

Synthesis and Application of Activity-Based Probes for Serine Proteases

  • Alba Ramos-Llorca, Valeria Parravicini, Koen Augustyns, Michaela Prothiwa

Pages 217-230

CellEKT: A Chemical Proteomics Workflow to Profile Cellular Target Engagement of Kinase Inhibitors – A Step-by-Step Guide

  • Berend Gagestein, Joel Rรผegger, Antonius P. A. Janssen, Stephan Kirchner, Uwe Grether, Arne C. Rufer et al.

Pages 231-253

Isolation of Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis

  • Yao Wang

Pages 255-264

Identification of Anticancer ROS Targets by Cysteine Reactivity Protein Profiling

  • Junbing Zhang, Liron Bar-Peled

Pages 265-273

A Comprehensive Data Processing Pipeline for Phosphoproteomics in Viral Infection Studies

  • Sini Huuskonen, Xiaonan Liu, Kari Salokas, Antti Tuhkala, Salla Keskitalo, Markku Varjosalo

Pages 275-291

Activity-Based Protein Profiling of Bacterial Monooxygenases

  • John B. Joyce, Michael R. Hyman

Pages 293-317

Activity-Based Profiling of Reactive Nucleophilic Cysteine Sites from Tissue Proteomes for Drug Discovery Applications

  • Hasini Kalpage, An Chi, Smaranda Bodea

Pages 319-329

Activity-Based Protein Profiling for Functional Cysteines and Protein Target Identification

  • Tin-Yan Koo, Clive Yik-Sham Chung

Pages 331-344

System-Wide Profiling of Ubiquitin E3 Ligase Activities with Quantitative Proteomics and Bioinformatics Analysis

  • Yue Chen, Yao Gong

Pages 345-359

In Part 10 of this 10-part series, intelligent claims from DeepSeek AI are pitted against the moronic claims of Grok AI.


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