Predicting the CRISPR Cas9 Market Collapse
Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo Knowing that nucleosomes interfere directly with Cas9 cleavage, but not with ZFN cleavage, is useful information. It may explain some cases of unanticipated sgRNA failure, and it should guide specific applications where precisely located, high-efficiency cleavage is necessary. Reported as: CRISPR’s hedgehog problem: Rolled-up genes can’t be … Predicting the CRISPR Cas9 Market Collapse