RNA-mediated gene silencing

Optoribogenetic-driven sympatric speciation (1)

For presentation during session: Polycomb and chromatin remodeling Historical perspective: Yet another kind of epigenetic imprinting occurs in species as diverse as yeast, Drosophila, mice, and humans and is based upon small DNA-binding proteins called “chromo domain” proteins, e.g., polycomb. These proteins affect chromatin structure, often in telomeric regions, and thereby affect transcription and silencing of various genes… Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at…

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UV light, H2O, and epigenetic effects… (1)

See first: Coherently organized healthy longevity (4) UV light, H2O, and epigenetic effects affect behavior and sympatric speciation Submitted by James V. Kohl on 9/25/20 for presentation during the Epigenetics – From Bench to Clinic session: RNA modifications and epitranscriptomics​​ Moderator Tony Kouzarides Title: UV light, H2O, and epigenetic effects affect behavior and sympatric speciation James V. Kohl* MicroRNA.pro Introduction UV light-activated, H2O-dependent oxidative phosphorylation links microRNA-mediated epigenetic effects on organized genomes from bench to clinic.  For a historical perspective,…

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pH-dependent healthy longevity (1)

Who else linked pH-dependent viral latency to pH-dependent healthy longevity via the Korean Genome Project‘s ethnicity-specific reference genome sequences? The facts about energy-dependent microRNA-mediated biophysically constrained viral latency can be compared to ridiculous theories from the gene-centric Human Genome Project touted by NIH Director, Francis S. Collins. Other neo-Darwinian theorists also failed to start with facts about God’s Creation of UV light and water, which Charles Darwin included in his energy-dependent “conditions of life” Darwin’s “conditions of life” presciently linked…

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pH-dependent viral latency (2)

New vaccines protect against COVID-19 in non-human primates, study finds 5/20/20 See for comparison: Vaccines do not control pandemics (1) 5/15/20 I reiterate: Emergence of Human G2P[4] Rotaviruses in the Post-vaccination Era in South Korea: Footprints of Multiple Interspecies Re-assortment Events 4/18/18 Amino acid substitutions at these positions were found to be associated with an inability to serotype a strain and are responsible for antigenic changes48. Recently, amino acid substitutions in the three main antigenic regions, especially at positions 96…

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The tipping point (revisited): 102K (1)

See first:  The tipping point (revisited): 101K (1) 4/22/20 and Vaccines do not control pandemics (1) 5/15/20 Genetic inactivation of the translin/trax microRNA-degrading enzyme phenocopies the robust adiposity induced by Translin (Tsn) deletion 5/11/20 They linked virus-driven changes in light-activated energy-dependent differences in hydrogen-atom transfer in DNA base pairs in solution from microRNA flanking sequences to the degradation of messenger RNA and all obesity-linked diseases via changes in the potential of hydrogen (pH). See: Control of pH responsive peptide self-association…

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Trump-hating advocates of vaccines + GMOs (2)

Trump-hating advocates of vaccines + GMOs (1) Conclusion: People who understand the facts about how energy-dependent ecological adaptations to viruses across kingdoms and across generations prevent pathology do not need more theoretical models. For instance, see: Genome-Wide Study Reveals a Novel Regulatory Pathway: Translation Affects mRNA Stability in a Codon-Dependent Manner on June 26, 2019. It is time to force theorists to move forward. Trump-hating advocates of vaccines + GMOs (2) For an attempt to return to the neo-Darwinian pseudoscientific…

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Natural models inspire future therapeutics (2)

Summary: Food odors and pheromones biophysically constrain viral latency, and an effective treatment for all neurodegenerative diseases was proposed in this 2011 patent application “Pheromones and the luteinizing hormone for inducing proliferation of neural stem cells and neurogenesis“ See for comparison: Identification of miR-379/miR-656 (C14MC) cluster downregulation and associated epigenetic and transcription regulatory mechanism in oligodendrogliomas. The post- transcriptionally regulatory network of this cluster showed enrichment of key cancer-related biological processes such as cell adhesion and migration. Also, there was…

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NHEJ disruption and HDR correction?

International Workshop “Targeting RNA world” Dear colleagues! Scientists of the Institute of Chemical Biology and Fundamental Medicine SB RAS cordially invites you to attend International Workshop “Targeting RNA world” in the frame of the project of Russian Scientific Foundation “New generation of RNA-targeting oligonucleotide derivatives as a platform for designing innovative therapeutics” (no. 14-44-00068). Workshop is being held in Park Inn by Radisson Pulkovskaya Hotel & Conference Centre St Petersburg, Saint-Petersburg, Russia, September 2-7 2018. We will discuss all aspects…

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Virus-driven genome engineering causes cancer?

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response Inhibition of p53 prevents the damage response and increases the rate of homologous recombination… In the 1990’s, Abcam researchers discovered the cell death gene. Now see: Mechanisms of Recombination conference See also: P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cells We demonstrated the toxic response is tp53-dependent and the toxic effect of tp53 severely reduces the efficiency of precise genome-engineering in hPSCs. Reported as:…

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An evolutionary theory killer

The concept of a species (3)

Gene expression evidence for remodeling of lateral hypothalamic circuitry in cocaine addiction (2005) This remodeling of LH circuitry could contribute to the chronic deficit in reward function that has been hypothesized to drive the transition to drug addiction. Lena D. van der Stap discussed her role in the publication of this article, which failed to make any assumptions about mutations and/or evolution. James Kohl Did you co-author Gene expression evidence for remodeling of lateral hypothalamic circuitry in cocaine addiction McEwen told…

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