Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

Reported as:

In vivo CRISPR-Cas9 screen sheds light on cancer metastasis and tumor evolution

Excerpt: “Unexpectedly, the screen also implicated several microRNAs — small RNA segments that are functional in the cell.
More experimental work remains to fully explore the genes and microRNAs uncovered in the screen.”

It is the perturbed balance of viral microRNAs and nutrient-dependent microRNAs that obviously makes the difference between RNA-directed DNA methylation and RNA-mediated amino acid substitutions that stabilize DNA in organized genomes and mutations linked to instability and to undifferentiated cell type proliferation, which is reported in the context of cancer. See for instance: Nutrient-dependent pheromone-controlled ecological adaptations: from atoms to ecosystems

Excerpt:

Experimental evidence continues to add support for the role of ecological variation and nutrient-dependent epigenetically-effected ecological adaptations that occur via amino acid substitutions, which determine the cell types of individuals in all species. More substantial support for epigenetic effects on cell type differentiation comes from what has been learned during the past decade about the role of small non-coding RNA molecules. The small non-coding RNA molecules are called microRNAs (miRNAs). MiRNAs alter intercellular signaling by changing the balance between miRNAs and messenger RNA (mRNA) . The changes are linked to health and to pathology (Mori et al., 2014).

If not for the claims of evolutionary theorists, serious scientists would long ago have learned the difference between nutrient-dependent amino acid substitutions and stress-linked mutations. Instead, evolutionary theory has been killing people with ignorance for more than 50 years. Theorists have ignored everything learned about physics, chemistry, and molecular biology because most of them believe in beneficial mutations.

They do not believe that the hemoglobin S variant is a nutrient-dependent ecological adaptation despite Dobzhansky’s mention that “Ingram and others found that hemoglobin S differs from A in the substitution of just a single amino acid, valine in place of glutamic acid in the beta chain of the hemoglobin molecule.” (1964)

Instead, many evolutionary theorists may believe that “…genomic conservation and constraint-breaking mutation is the ultimate source of all biological innovations and the enormous amount of biodiversity in this world.” Nei (2013) Most theorists have been taught not to challenge the teachings of their biologically uninformed professors, and they become biologically uninformed researchers. What we see here is the beginning of research results that report facts like this: “Life is physics and chemistry and communication.” Witzany (2014)

Uncontrolled viral microRNAs perturb the communication via mutations. Nutrient-dependent microRNAs facilitate communication via the RNA-mediated control of viral microRNAs by amino acid substitutions.

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