Active Motif claims that Active Motif is the industry leader in developing and delivering innovative tools to enable epigenetics and gene regulation research.

See how the role of light is portrayed in LightSwitch™ Luciferase Assay System (with my emphasis)

Excerpt 1) Combined with our large collection of miRNA Mimics and Inhibitors, you have everything needed to study miRNA-3´UTR interactions, validate miRNA targets, measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis.

Excerpt 2) The LightSwitch lncRNA Promoter Reporter Collection was designed to make it fast & easy to study the regulation of lncRNA promoters.

Excerpt 3) LightSwitch™ Validated Pathway Collections… have been experimentally validated to show significant activation or repression in response to pathway-specific induction conditions, so they are ideal when studying pathways such as inflammation, hypoxia, DNA damage, heat shock, etc.

My comment: They portray the light energy-dependent active motifs as switches. The switching is RNA-mediated. Fixation of RNA-mediated amino acid substitutions links the biophysically constrained chemistry of protein folding from the innate immune system to supercoiled DNA via the physiology of energy-dependent reproduction in all living genera.

The facts about “…everything needed to study miRNA-3´UTR interactions, validate miRNA targets, measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis” were included in this invited review of nutritional epigenetics. Active Motif repeatedly attests to the experimental evidence that attests to the facts in:

Nutrient-dependent pheromone-controlled ecological adaptations: from atoms to ecosystems

Abstract:

This atoms to ecosystems model of ecological adaptations links nutrient-dependent epigenetic effects on base pairs and amino acid substitutions to pheromone-controlled changes in the microRNA / messenger RNA balance and chromosomal rearrangements. The nutrient-dependent pheromone-controlled changes are required for the thermodynamic regulation of intracellular signaling, which enables biophysically constrained nutrient-dependent protein folding; experience-dependent receptor-mediated behaviors, and organism-level thermoregulation in ever-changing ecological niches and social niches. Nutrient-dependent pheromone-controlled ecological, social, neurogenic and socio-cognitive niche construction are manifested in increasing organismal complexity in species from microbes to man. Species diversity is a biologically-based nutrient-dependent morphological fact and species-specific pheromones control the physiology of reproduction. The reciprocal relationships of species-typical nutrient-dependent morphological and behavioral diversity are enabled by pheromone-controlled reproduction. Ecological variations and biophysically constrained natural selection of nutrients cause the behaviors that enable ecological adaptations. Species diversity is ecologically validated proof-of-concept. Ideas from population genetics, which exclude ecological factors, are integrated with an experimental evidence-based approach that establishes what is currently known. This is known: Olfactory/pheromonal input links food odors and social odors from the epigenetic landscape to the physical landscape of DNA in the organized genomes of species from microbes to man during their development.

My comment: Claims by Active Motif’are parroted by others. The facts can be linked from the LightSwitch™ Validated Pathway Collections to the study of pathways that link nutritional epigenetics to prevention of inflammation, hypoxia, DNA damage, heat shock, et al., via changes in pH. Femtosecond blasts of ultraviolet (UV) light link the changes in pH link from hydrogen-atom transfer in DNA base pairs in solution to RNA-mediated DNA repair in all living genera.

For example, all living genera reproduce. That’s a fact! That fact links the Laws of Biology in my model to all biodiversity. The Laws of Biology refute every aspect of neo-Darwinian theory with experimental evidence of biologically-based cause and effect. The facts were revisited in a series of presentations during Labroots virtual conferences. But remember this claim:

…everything needed to study miRNA-3´UTR interactions, validate miRNA targets, measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis…

The experimentally validated facts were linked from energy-dependent changes in angstroms to ecosystems in these 4 presentations.

The Origin of Information: How to Solve It

video

From hydrogen atom transfer in DNA base pairs to ecosystems

video

What is life when it is not protected from virus driven entropy

video

RNA-mediated physics, chemistry, and molecular epigenetics

video

For an easy to understand representation of facts that link angstroms to ecosystems in all living genera via nutrient energy-dependent supercoiled DNA, see:

For comparison, see: Active Motif Webinars

Excerpt:

Stay up-to-date with the latest tips & techniques from our Epigenetics experts.

My comment: If you find any information that suggests the representations at RNA-mediated.com are inaccurate or that the representations on the RNA-mediated FB group  are not supported by experimental evidence of biologically-based cause and effect, please comment on what you found for comparison Alternatively, watch for the results of the patent battle that will result if this patent application is successful.

See: RNA-Guided Human Genome Engineering reported in the context of this Secret meeting

Excerpt:

Why would a bunch of scientists need to exclude the media and the public from a meeting about something as ethically fraught as synthesizing a human genome?

The only reason to exclude anyone who could possible challenge the representations made at the secret meeting is to keep secrets about the facts. Those who keep secrets about energy-dependent RNA-mediated cell type differentiation have help from people who know nothing about the secrets that are being kept.

See also:  Evolution, Mutation, Super Bacteria, Oh My: When Mr. Jerry Coyne Shoots Himself in the Foot

September 14, 2016 Nathan van Ree wrote: Just to get it clear: do I understand correctly that Tomi got knowledge from your publication, misrepresented your publication by cherry picking parts of it and post those here, which made your presentation look so bad people outside of this group decided not to publish it?

What’s the difference between your conclusions and the way Tomi represents them and why would he do that?

Are your conclusions against the position of YEC, that of (neo) Darwinism, or neither?

My response: Thanks for helping to clarify what he has done, before asking. Tomi has only done the cherry-picking after the fact, and after publication of my 2013 review. Others cherry picked the information in the invited review that was returned without review.

The difference is the amount of secrecy used to mislead others who might decide to accept Tomi’s beliefs compared to the experimental evidence of biologically-based cause and effect. My conclusions are based on facts, which link my publication history across twenty years to the published works of other serious scientists.

See also the secretive approach by antagonist Tomi Aalto, who others seem to think is supporting my model with his plagiarism.

Mechanisms known to affect the phenotype

1. RNA methylation
2. DNA dinucleotide methylation
3. DNA CpG island methylation
4. Histone methylation
5. Chromatin remodeling
6. DNA coiling
7. MicroRNA regulation
8. Alternative splicing

All phenotypes are nutrient-energy dependent and virus-driven energy theft causes all pathology. In our 1996 Hormones and Behavior review, we wrote:

Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at least two species, Drosophila melanogaster and Caenorhabditis elegans (Adler and Hajduk, 1994; de Bono, Zarkower, and Hodgkin, 1995; Ge, Zuo, and Manley, 1991; Green, 1991; Parkhurst and Meneely, 1994; Wilkins, 1995; Wolfner, 1988). That similar proteins perform functions in humans suggests the possibility that some human sex differences may arise from alternative splicings of otherwise identical genes.

The pre-mRNAs link energy-dependent changes in the microRNA/messenger RNA balance to all biodiversity, which is the next thing that Tomi Aalto will ineffectively try to do. Find out where Tomi got his information on energy-dependent RNA methylation and how he linked it to energy-dependent alternative splicings without mention of nutrient energy-dependent changes in hydrogen-atom transfer in DNA base pairs that link the physiology of reproduction to fixation of RNA-mediated amino acid substitutions in all organized genomes via supercoiled DNA, which protects all species from virus-driven energy theft and genomic entropy.

Genomic entropy is biophysically constrained by energy, not by evolution.

See for comparison: Brain evolution and development: adaptation, allometry and constraint

Excerpt:

2. What explains the presence of genetic correlations? Where present, the strength of genetic correlations between components could be combined with data on developmental (or evolutionary) origin and connectivity, to test whether genetic correlations evolve in response to functional integration (figure 1, scenario (v)), or reflect patterns of conserved developmental origin (figure 1, scenario (i)).

My comment: Energy-dependent RNA-mediated biophysically constrained protein folding explains genetic correlations, which arise only in the context of detailed links from the innate immune system to supercoiled DNA. Energy-dependent changes are linked to all cell type differentiation. Claims about evolution are the antithesis of claims that link ecological variation to energy-dependent ecological adaptations via what is known to all serious scientists about energy-dependent RNA-mediated protein folding chemistry.

See for comparison: Mutation-Driven Evolution

…genomic conservation and constraint-breaking mutation is the ultimate source of all biological innovations and the enormous amount of biodiversity in this world. In this view of evolution there is no need of considering teleological elements” (p. 199).

See also: Alternative RNA Splicing in Evolution

It now appears that alternative splicing is, perhaps, the most critical evolutionary factor determining the differences between human beings and other creatures.

My comment: Alternative RNA splicing is not an evolutionary factor. It links energy-dependent changes from angstroms to ecosystems in all living genera via fixation of RNA-mediated amino acid substitutions in the context of the physiology of reproduction, which is controlled by pheromones in species from microbes to humans.

From Fertilization to Adult Sexual Behavior

Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at least two species, Drosophila melanogaster and Caenorhabditis elegans (Adler and Hajduk, 1994; de Bono, Zarkower, and Hodgkin, 1995; Ge, Zuo, and Manley, 1991; Green, 1991; Parkhurst and Meneely, 1994; Wilkins, 1995; Wolfner, 1988). That similar proteins perform functions in humans suggests the possibility that some human sex differences may arise from alternative splicings of otherwise identical genes.

 See also: Circular RNAs: Novel Regulators of Neuronal Development

 

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