physiology of reproduction

God’s shrinking role in salvation (2)

Conclusion: There is no such thing as a blind dance of atoms. Quantized energy-dependent changes link angstroms to ecosystems in all living genera via the physiology of reproduction. Serious scientists do not link “high-level cause and purposes” to anything that does not link what organisms eat from food energy to the physiology of reproduction. Only pseudoscientists claim that ideas about “emergence” are “…a remarkable synthesis across fields and levels.” See: God’s shrinking role in salvation These 6 Common Vegetables Are…

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Hard Science vs the neo-Darwinian “magic box”

Summary: Identical nucleotide sequences link energy-dependent changes in binding sites to different amino acids in different proteins. The binding sites are conserved in the orthologous mammalian genes. The in vivo structure of biological membranes and evidence for lipid domains Scientists investigate how the sense of smell works in bacteria Biogenic non-crystalline U(IV) revealed as major component in uranium ore deposits As presciently predicted in the works of Richard Feynman and Bruce McEwen, bacteria feed on something that allows them to…

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Energy-dependent epigenetic translation to mRNA stability (6)

See: Energy-dependent epigenetic translation to mRNA stability (5) Five energy-dependent amino acid substitutions are linked to differences in RNA content and biodiversity in 4 mammalian species. Excerpt: Species- and tissue-specific variation in mt-tRNA incorporation into the mt-LSU was analyzed….  Analysis of porcine dermal fibroblasts (C), human osteosarcoma 143B cells (D), bovine cardiac tissue (E), and rat hepatic tissue (F) depicts the relative abundance of each mt-tRNA across fractions (ImageQuant software); represented as a percentage of the total signal. In my…

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Energy-dependent epigenetic translation to mRNA stability (5)

Energy-dependent epigenetic translation to mRNA stability (4) From Fertilization to Adult Sexual Behavior Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at least two species, Drosophila melanogaster and Caenorhabditis elegans…. See also: The Supraspliceosome – A Multi-Task Machine for Regulated Pre-mRNA Processing in the Cell Nucleus Abstract conclusion: …changes in these regulatory processing activities are associated with human disease and cancer. These findings emphasize the supraspliceosome as…

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Energy as information and constrained endogenous RNA interference (4)

Premise: Sunlight is the energy as information that is required to link endogenous RNA interference to healthy longevity Counter claim:  The Quantum Thermodynamics Revolution: Information theory Understanding entropy as a subjective measure allows the universe as a whole to evolve without ever losing information. Precision Medicine virtual conference, Feburary 22-23, 2017 Title: Energy as information and constrained endogenous RNA interference  James V. Kohl RNA-mediated.com jvkohl@bellsouth.net Abstract describing the poster   Narrative: The term microRNA has been used in 58,000 indexed…

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Virus-driven downsizing of the human brain (4)

Virus-driven downsizing of the human brain (7/1/16) Virus-driven downsizing of the human brain (2) (7/6/16) Virus-driven downsizing of the human brain (3) (8/9/16) Excerpt: A Postdoctoral Researcher threatened me with litigation if I did not remove the tags with his name that linked his works to works at the institution where he works. In an attempt to discuss Breakthrough in understanding how stem cells become specialized More than two years ago, he wrote: We house our animal mouse models in…

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Viruses in pathogenic variants disrupt alternative splicings

Pathogenic variants that alter protein code often disrupt splicing Approximately 10% of exonic mutations altered splicing, mostly by disrupting multiple stages of spliceosome assembly. We present a large-scale characterization of exonic splicing mutations using a new technology that facilitates variant classification and keeps pace with variant discovery. Assay of nearly 5,000 mutations reveals roots of genetic splicing errors “Splicing errors can be very deleterious because instead of just changing one amino acid [the building block of a protein], it can…

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Epigenetically-effected nucleosome repositioning sheds Dobzhansky’s light on evolution

Crystal structure of the overlapping dinucleosome composed of hexasome and octasome My summary: Nucleosomes are dynamic entities that are repositioned by energy-dependent links from sunlight to ATP and RNA biosynthesis that act on DNA during the chromatin remodeling, which is biophysically constrained by the pheromone-controlled physiology of reproduction during the transgenerational epigenetic inheritance of all morphological and behavioral phentoypes. processes. Reported as: Unveiling how nucleosome repositioning occurs to shed light on genetic diseases …an incomplete formation of overlapping dinucleosome may…

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Synthetic RNA-based switches

Synthetic RNA-based switches for mammalian gene expression control Current Opinion in Biotechnology, Volume 48, December 2017, Pages 54–60 If you wait until December, 2017 to see this article in print, you will probably be among the last of those to have missed a two decades-old paradigm shift. The fact that these researchers created synthetic RNA-based switches attests to the fact that natural selection for energy-dependent codon optimality links what organisms eat to energy-dependent RNA-mediated amino acid substitutions. The substitutions help…

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Functionally interdependent editing and methylation

Editing and methylation at a single site by functionally interdependent activities …cytosine 32 in the anticodon loop of Trypanosoma brucei tRNAThr is methylated to 3-methylcytosine (m3C) as a pre-requisite for C-to-U deamination. Formation of m3C in vitro requires the presence of both the T. brucei m3C methyltransferase TRM140 and the deaminase ADAT2/3. Once formed, m3C is deaminated to 3-methyluridine (m3U) by the same set of enzymes. ADAT2/3 is a highly mutagenic enzyme4, but we also show that when co-expressed with…

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