RNA-Guided Human Genome Engineering

Virus-driven genome engineering causes cancer?

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response Inhibition of p53 prevents the damage response and increases the rate of homologous recombination… In the 1990’s, Abcam researchers discovered the cell death gene. Now see: Mechanisms of Recombination conference See also: P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cells We demonstrated the toxic response is tp53-dependent and the toxic effect of tp53 severely reduces the efficiency of precise genome-engineering in hPSCs. Reported as:…

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Food energy-dependent epigenetic adaptation

Mechanism of transmembrane signaling by sensor histidine kinases, was reported as Scientists investigate how the sense of smell works in bacteria …the signaling and inactive states differ only very slightly at the nitrate-binding site – by 0.5-1 angstroms, which is approximately one fifth of the size of the ion itself (1 angstrom is 10-10 meters). However, when this ion binds to the sensor, it causes huge changes in the protein: The helices of different monomers begin to move in different…

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Energy-dependent epigenetic translation to mRNA stability (6)

See: Energy-dependent epigenetic translation to mRNA stability (5) Five energy-dependent amino acid substitutions are linked to differences in RNA content and biodiversity in 4 mammalian species. Excerpt: Species- and tissue-specific variation in mt-tRNA incorporation into the mt-LSU was analyzed….  Analysis of porcine dermal fibroblasts (C), human osteosarcoma 143B cells (D), bovine cardiac tissue (E), and rat hepatic tissue (F) depicts the relative abundance of each mt-tRNA across fractions (ImageQuant software); represented as a percentage of the total signal. In my…

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Who will kill the billion dollar baby? Bill Gates or Donald Trump

Bill Gates is set to meet with the President on Monday. [March 20, 2017] Both men know that virus-driven energy theft is the biggest threat humanity has ever faced. The threat arose in the context of wasted US government funding and wasted funding from philanthropists who supported the CRISPR gene-editing technology. For comparison, The Penrose Institute appears to be prepared to establish itself as a refuge for serious scientists who have been cast out of academia for their failure to…

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Energy-dependent pheromone-controlled entropy (2)

See also: Energy-dependent pheromone-controlled entropy (1) Let’s make peer review scientific …peer review is often biased and inefficient. It is occasionally corrupt, sometimes a charade, an open temptation to plagiarists. Even with the best of intentions, how and whether peer review identifies high-quality science is unknown. It is, in short, unscientific. The androgynous baby-faced boy or girl pictured among those wearing pink “crotch” hats appears to representing others at the March for Women. The sign may also be representing the…

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Energy-dependent pheromone-controlled entropy (1)

Let’s make peer review scientific …peer review is often biased and inefficient. It is occasionally corrupt, sometimes a charade, an open temptation to plagiarists. Even with the best of intentions, how and whether peer review identifies high-quality science is unknown. It is, in short, unscientific. A recent PubMed search for more peer-reviewed articles that mention microRNA returned a link to this published work. “Genetic 3’UTR variation is associated with human pigmentation characteristics and sensitivity to sunlight” One energy-dependent variation links…

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The essence of precision medicine

The media-industrial complex attack CNN chief: Politicians should oppose Trump’s attack on media …Trump’s labeling of the media as the enemy of the state was unfortunate and dangerous. It was an accurate label that has been supported by Bill Gates and the Allen Institute in the context of the forthcoming viral apocalypse. See for comparison this enemy of the state-issued media representation:   “We are all proud of professor Filipp’s accomplishments,” Vice Chancellor for Research Samuel Traina said. “He exemplifies…

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Polycombic ecological adaptation as a science, not a theory

Multi-omic data integration enables discovery of hidden biological regularities   Excerpt: Tracing the central dogma of biology (left column), we can link specific data types (middle column) to explain each of these biological processes. In this work, novel biological regularities that relate these processes are discovered through: (i) primary omics data (top box, right column) and (ii) integration with GEMs (bottom box, right column).   Excerpt: The approach directly addresses the BD2K grand challenge and is made conceptually accessible by…

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Hypothesis free pseudoscience vs facts (3)

Nobel-Winning DNA Research Challenges Evolutionary Theory In 2015, three scientists won the Nobel Prize in Chemistry for decades of research into DNA—research that reinforces the idea that evolution is mythology and makes the modern evolutionary theory of abiogenesis seem more and more indefensible. It turns out that DNA is inherently unstable, and the preservation of genetic information requires a complex symbiotic relationship between the cell and DNA that is so interdependent that neither could have arisen independently of the other.…

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Chromatin: The structure of DNA

Update on Inflammation and Degenerative Brain Disease Excerpt: While mapping connections of neurons has become the holy grail of current neuroscience, it is clear that communication of neurons with many other types cells is, also, vitally important to every aspect of brain function. My comment: Understanding dementia was placed into the context of understanding how virus-driven energy theft alters the de novo creation of olfactory receptor genes and causes the loss of function that is linked to all pathology via…

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