RNAi

Coherently organized healthy longevity (1)

A large-scale binding and functional map of human RNA-binding proteins [RBPs] 7/29/20 We describe the spectrum of RBP binding throughout the transcriptome and the connections between these interactions and various aspects of RNA biology, including RNA stability, splicing regulation and RNA localization. These data expand the catalogue of functional elements encoded in the human genome by the addition of a large set of elements that function at the RNA level by interacting with RBPs. Reported as: ENCODE consortium identifies RNA…

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The microRNA-mediated future of humanity (8)

The levels of complexity linked to the microRNA-mediated future of humanity can be examined by ages 10-14+ via game-play of: Bacillus (failed project) See instead: Function of miR825 and miR825* as Negative Regulators in Bacillus cereus AR156-elicited Systemic Resistance to Botrytis cinerea in Arabidopsis thaliana 10/17/19 Peptide Players compete to link amino acids to build a peptide chain (a fancy word for a protein). Virulence Players take on the role of viruses and compete to infect a host cell in…

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Autophagy is the antiphage defense strategy (2)

2/15/17 Energy as information and constrained endogenous RNA interference (video) The 6.46 minute-long technical presentation (above) from the Precision Medicine Virtual Conference is difficult to understand.Thus, 10 months later, Nik Willmore asked: 12/24/17 What are your insights into CRISPR and mTOR, for a young audience? I’m not mocking you…yet, Humpty Dumpty. What is the basis of your unifying principle? Vibration? Bohmian morphogenetic fields? Modified Darwin? God? –Nik Willmore @nikwillmore 12/25/17 Replying to @nikwillmore @jhewitt123 God created the anti-entropic virucidal energy of sunlight and…

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Quantized energy-dependent viral trophism (3)

Summary: If the pathway is ancestral, how and when did it evolve to protect species from microbes to humans from the virus-driven degradation of their messenger RNA? Peer-Reviewed Science: A “Mathematical Proof of Darwinian Evolution” Is Falsified The theorem addresses only the effects of natural selection. Fisher did not directly address any other effect (mutation, genetic drift, environmental change, etc.) as he considered them to be insignificant. Later mathematicians took issue with Fisher’s lack of rigor, some at considerable length.…

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For God and Country

Sci-Hub vs limits on the dissemination of information

The ideology of “Americanism” is not consistent with the tactics of academics and politicians that constrain release of accurate information about  biologically-based cause and effect. …Americanism is an ideology, or belief in devotion, loyalty, or allegiance to the United States of America, or to its flag, traditions, customs, culture, symbols, institutions, or form of government.[2] In the words of Theodore Roosevelt, “Americanism is a question of spirit, conviction, and purpose, not of creed or birthplace.”[3] Americanism has two different meanings.…

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The billion dollar baby (revisited)

Summary: All energy-dependent differences in the microRNA/messenger RNA balance have now been linked from the physiology of pheromone-controlled reproduction to all morphological and behavioral phenotypes by the conserved molecular mechanisms of RNA-mediated cell type differentiation. FDA Approves Treatment That Modifies Patient’s Own Genes To Fight Cancer It costs $475,000 and the treatment could kill you. Discussion attempt  Keep in mind that you could have stopped this. Now, you either continue to play their game, or play the cell biology game…

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Epigenetically-effected nucleosome repositioning sheds Dobzhansky’s light on evolution

Crystal structure of the overlapping dinucleosome composed of hexasome and octasome My summary: Nucleosomes are dynamic entities that are repositioned by energy-dependent links from sunlight to ATP and RNA biosynthesis that act on DNA during the chromatin remodeling, which is biophysically constrained by the pheromone-controlled physiology of reproduction during the transgenerational epigenetic inheritance of all morphological and behavioral phentoypes. processes. Reported as: Unveiling how nucleosome repositioning occurs to shed light on genetic diseases …an incomplete formation of overlapping dinucleosome may…

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Functionally interdependent editing and methylation

Editing and methylation at a single site by functionally interdependent activities …cytosine 32 in the anticodon loop of Trypanosoma brucei tRNAThr is methylated to 3-methylcytosine (m3C) as a pre-requisite for C-to-U deamination. Formation of m3C in vitro requires the presence of both the T. brucei m3C methyltransferase TRM140 and the deaminase ADAT2/3. Once formed, m3C is deaminated to 3-methyluridine (m3U) by the same set of enzymes. ADAT2/3 is a highly mutagenic enzyme4, but we also show that when co-expressed with…

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Wikipedia refutes theistic evolution

RNA-Directed DNA Methylation Besides RNA molecules, a plethora of proteins are involved in the establishment of RdDM, like Argonautes, DNA methyltransferases, chromatin remodelling complexes and the plant-specific Polymerase IV and Polymerase V. All these act in concert to add a methyl-group at the 5′ position of cytosines. In contrast to animals, cytosines at all sequence context (CG, CHG, CHH) may get de novo methylated in plants. There is no such thing as de novo methylation. Methylation is energy-dependent. Virus-driven energy…

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The natural success of RNAi and failed treatment

see my other blog posts on m6A Dynamics of Human and Viral RNA Methylation during Zika Virus Infection Excerpt: …ZIKV infection alters m6A location in mRNAs, methylation motifs, and target genes modified by methyltransferases. Our results identify a mechanism by which ZIKV interacts with and alters host cell functions. Reported as: Zika virus infection alters human and viral RNA Excerpt: When Zika virus infects a human cell, Rana’s team found, the cell modifies viral RNA with m6A as a means…

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