stochastic gene expression

Impeaching the God of Abraham (9)

In “Impeaching the God of Abraham (7) I linked achiral glycine in position 6 of the GnRH decapeptide from peptide synthesis at the origin of life to the patent for naturally occurring light-activated carbon fixation and protonated RNA interference. See also: Regulatory Mechanisms of Vitellogenesis in Insects 1/28/21 …microRNA (miRNA) and nutritional (amino acid/Target of Rapamycin and insulin) pathways interplay with JH and 20E signaling cascades to control insect vitellogenesis. This article is part of the Research Topic Germ Cell…

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Impeaching the God of Abraham (5)

More than 116,000 indexed published works link sunlight from microRNA biogenesis to biophysically constrained viral latency across kingdoms via the energy-dependent assembly of the microRNA-RNA-peptide nanocomplex. But see: Experts say there’s no medical basis for Trump’s suggestion that sunlight, disinfectants may treat coronavirus 4/24/21 Jonathan Reiner, professor of medicine at The George Washington University Hospital, said there’s no medical basis for Trump’s statements about sunlight. See for comparison: Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction (1986)…

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Neurosteroids revisited two decades later

See also: Host-derived creation of all pathology (2 of 2) These two statements are not being linked to the prevention of all disease. 1) “The synthesis of RNA in isolated thymus nuclei is ATP dependent.” (1964) 2) “Nucleosome remodelling (NR) regulates transcription in an ATP-dependent manner…” (2017) See: Cell-targetable DNA nanocapsules for spatiotemporal release of caged bioactive small molecules The link from olfaction to the space-time continuum via energy-dependent endogenous RNA interference was reported as: Tiny nanopackages built out of…

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Energy-dependent physical and biophysical constraints (7)

See first: Energy-dependent physical and biophysical constraints (6) Stems Cells in the Hypothalamus Slow Aging in Mice My comment to The Scientist Cai says microRNAs could be a potential mechanism by which hypothalamic neural stem cells have such wide-ranging effects on aging, yet he believes that neurogenesis may also be involved. See also: Feedback loops link odor and pheromone signaling with reproduction At least 10,000 neurons in 26 different brain areas appear to transmit signals directly to GnRH neurons.  GnRH…

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Energy-dependent microRNA biogenesis

Published May 3, 2017 Genetic variation and RNA structure regulate microRNA biogenesis …primary sequence determinants and RNA structure are key regulators of miRNA biogenesis. Genetic variation aka primary sequence determinants are energy-dependent and RNA-mediated. Energy-dependent RNA methylation is an experience-dependent link from natural selection for energy-dependent codon optimality. Natural selection for energy-dependent codon optimality links ATP-dependent microRNA biogenesis to RNA biosynthesis. Ridiculous attempts to portray energy-dependent top-down causation as if genetic variation came first may continue for another 52 years.…

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Open Science: Closed to facts about microRNAs

See my 5-part series on: The tipping point? 50, 000 publications Imagine how we could accelerate innovation, stimulate economic growth and all the solutions we need to live on a healthy and sustainable planet, if we were to open up our science fully and allow a free flow of scientific knowledge.” says Kamila Markram, a neuroscientist and the CEO of Frontiers, one of the largest Open Access science publishers in the world and a leader in the Open Science movement.…

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The miRNA/mRNA balance: a suboptimal strategy?

Cells exercise suboptimal strategy to survive Excerpt: “Learning the secrets of alternate molecular pathways could provide clues about how to use them to patients’ advantage, she said.” My comment: The same molecular pathways control the nutrient-dependent physiology of reproduction in all genera. They link RNA-directed DNA methylation to RNA-mediated cell type differentiation via fixation of amino acid substitutions that determine cell types. See also: Epistasis Among Adaptive Mutations in Deer Mouse Hemoglobin “Mechanisms of epistasis are often best revealed through…

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Single-cell level assay of protein biosynthesis and degradation

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry Excerpt: “We use this technique to show modulation of a microRNA critical for T-cell function, miR-155. We adapt this assay for simultaneous detection of mRNA and proteins by ImageStream technology.” Reported as: Technology breakthrough reveals cellular transcription process My comment: Their assay simultaneously detects epigenetically-effected changes in the miRNA/mRNA balance and proteins, which link nutrient-uptake to the physical landscape of DNA in the organized genomes of…

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