The levels of complexity linked to the microRNA-mediated future of humanity can be examined by ages 10-14+ via game-play of:
Bacillus (failed project)
Peptide Players compete to link amino acids to build a peptide chain (a fancy word for a protein).
Virulence Players take on the role of viruses and compete to infect a host cell in order to replicate their own viral components allowing players to build their power or score points!
Subatomic Particle physics and chemistry collide
Periodic A Game of The Elements
Cytosis A Cell Biology Game (starts from the Creation of ATP)
Virus Expansion Player may pay ATP to build up their antibodies to each of the three viral strains.
Ecosystem Earn points by aligning animals with habitats where they most flourish.
With the availability of Genotype (October 2020) every mature intelligent person on Earth who is age 10-14+ can see that after three generations of light-activated biophysically constrained plant growth, neo-Darwinian theorists and Big Bang cosmologists lose their abilities to claim expertise. Why? Because no experimental evidence of top-down causation links God’s Creation of energy as information from the physiology of reproduction to the mutation-driven evolution of anything except more diseases!
The NS5B protein of the hepatitis C virus (HCV) is an RNA-dependent RNA polymerase (RdRp) (S.-E. Behrens, L. Tomei, and R. De Francesco, EMBO J. 15:12-22, 1996) that is assumed to be required for replication of the viral genome.
See for comparison: Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir (2020)
The N4 hydroxyl group off the cytidine ring forms an extra hydrogen bond with the side chain of K545 and cytidine base also forms an extra hydrogen bond with the guanine base from the template strand. These two extra hydrogen bonds may explain the apparent higher potency of EIDD-2801 in inhibiting SARS-CoV-2 replication.
The facts about hydrogen bond-dependent effective coronavirus treatment with remdesivir can now be linked to more than two decades of indexed published works that link what’s known about biophysically constrained viral latency from quantum coherence to coherently organized biology in these publications,
See: “RNA-dependent RNA polymerase” (items 1-4162)
The link from energy-dependent changes in base pairs to microRNA biogenesis and fixation of RNA-mediated amino acid substitutions requires even less investigation of top-down causation and the effect of anti-entropic UV light.
See: “RNA-dependent RNA polymerase” “amino acid” (items 1-1221)
Light-activated microRNA biogenesis has been linked from the energy-dependent Creation of RNA-dependent RNA polymerase via the Calvin Cycle and fixation of amino acid substitutions to biophysically constrained viral latency and healthy longevity in all living genera via the physiology of reproduction.
For example, see:
Single amino acid substitutions in the coat protein and RNA-dependent RNA polymerase alleviated the virulence of Cucumber green mottle mosaic virus and conferred cross protection against severe infection. Liu J et al. Virus Genes. (2020)
See also: Amino acid residues Ala283 and His421 in the RNA-dependent RNA polymerase of porcine reproductive and respiratory syndrome virus play important roles in viral ribavirin sensitivity and quasispecies diversity. Tian D et al. J Gen Virol. (2016)
What can neo-Darwinian theorists continue to claim without being ridiculed?
In 2003, Eugene Koonin and others found nothing that would place the origin of highly conserved RNA-dependent RNA polymerase, which missing in archaea and bacteria but is highly conserved in most eukaryotes, back into the context of neo-Darwinian pseudoscientific nonsense about the evolution of biodiversity across kingdoms.
The eukaryotic RNA-dependent RNA polymerase (RDRP) is involved in the amplification of regulatory microRNAs during post-transcriptional gene silencing. This enzyme is . No evolutionary relationship between RDRP and other polymerases has been reported so far, hence the origin of this eukaryote-specific polymerase remains a mystery.
We found that there are at least two evolutionarily distinct mechanisms that compensate for the absence of piRNAs, both involving RNA-dependent RNA polymerases (RdRPs).
“No evolutionary relationship between RDRP and other polymerases has been reported…” since 2003, but they claim to have found two two evolutionarily distinct mechanisms.
A mutation in RNA-dependent RNA polymerase 2, another essential gene for the biogenesis of endogenous 24-nt siRNAs, also rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation.
They found a good mutation and a bad mutation and linked both to microRNA-mediated biogenesis of RNA-dependent RNA polymerase 2. SARCASM ALERT: Nothing can stop evolutionary theorists from making claims that link mutations to good and bad outcomes, unless remdesivar in not really effective in stopping the replication of coronaviruses, such as Covid-19.
In plants, RNAi is also called post-transcriptional gene silencing (PTGS), in which diverse small RNAs are utilized to defend against viral or bacterial infection, and to modulate endogenous gene expression and chromatin structure.
United States Patent Application 20160002670 RNA-Guided Human Genome Engineering
FIG. 3A-1 and FIG. 3A-2 depict a human codon-optimized version of the Cas9 protein and full sequence of the cas9 gene insert.
Naturally occurring light-activated microRNA biogenesis links carbon fixation to biophysically constrained viral latency via fixation of amino acid substitutions in the context of the pheromone-controlled physiology of reproduction in species from microbes to humans.
5. Repetitive elements or endogenous viral elements can be targeted with engineered Cas+gRNA systems in microbes, plants, animals, or human cells to reduce deleterious transposition or to aid in sequencing or other analytic genomic/transcriptomic/proteomic/diagnostic tools (in which nearly identical copies can be problematic).
This was reported to be “A Billion Dollar Baby in Therapy.
“RNAi has targeted exogenous genes in models of viral infection…”
Effective treatment of coronavirus pathology with remdesivar failed to abort this baby. It’s been delivered at a cost of $4500 per treatment.
When you are talking about saving a life, that $4,500 “seems really reasonable,” Piper Sandler’s Tyler Van Buren said in a phone call.